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A novel activity for fungal nitronate monooxygenase: detoxification of the metabolic inhibitor propionate-3-nitronate.
- Source :
-
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2012 May; Vol. 521 (1-2), pp. 84-9. Date of Electronic Publication: 2012 Mar 21. - Publication Year :
- 2012
-
Abstract
- Nitronate monooxygenase (NMO; E.C. 1.13.12.16) oxidizes alkyl nitronates to aldehydes and nitrite. Although the biochemistry of the enzyme from fungal sources has been studied extensively, the physiological role is unknown. The ability of NMO to detoxify propionate-3-nitronate was tested by measuring growth of recombinant Escherichia coli containing the gene encoding for the enzyme in either the absence or presence of the nitronate and its conjugate acid 3-nitropropionate. The mixture propionate-3-nitronate/3-nitropropionate is toxic to E. coli cells lacking expression of NMO, but the toxicity is overcome through either induction of the gene for NMO or through addition of exogenous enzyme to the cultures. Both Williopsis saturnus and Neurospora crassa were able to grow in the presence of 0.4mM propionate-3-nitronate and 19.6mM 3-nitropropionate, while a knockout mutant of N. crassa lacking NMO was inhibited by concentrations of propionate-3-nitronate and 3-nitropropionate >0.3 and 600μM, respectively. These results strongly support the conclusion that NMO functions to protect the fungi from the environmental occurrence of the metabolic toxin.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Antimetabolites toxicity
Escherichia coli drug effects
Escherichia coli metabolism
Fungal Proteins genetics
Gene Knockout Techniques
Genes, Fungal
Kinetics
Metabolic Detoxication, Phase I
Neurospora crassa drug effects
Neurospora crassa enzymology
Neurospora crassa genetics
Neurospora crassa growth & development
Nitro Compounds toxicity
Oxidoreductases genetics
Propionates toxicity
Recombinant Proteins genetics
Recombinant Proteins metabolism
Substrate Specificity
Superoxides metabolism
Williopsis enzymology
Williopsis genetics
Antimetabolites metabolism
Fungal Proteins metabolism
Nitro Compounds metabolism
Oxidoreductases metabolism
Propionates metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0384
- Volume :
- 521
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Archives of biochemistry and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 22464989
- Full Text :
- https://doi.org/10.1016/j.abb.2012.03.015