Back to Search
Start Over
Fingerprint-imprinted polymer: rational selection of peptide epitope templates for the determination of proteins by molecularly imprinted polymers.
- Source :
-
Analytical chemistry [Anal Chem] 2012 May 01; Vol. 84 (9), pp. 4036-41. Date of Electronic Publication: 2012 Apr 17. - Publication Year :
- 2012
-
Abstract
- The pool of peptides composing a protein allows for its distinctive identification in a process named fingerprint (FP) analysis. Here, the FP concept is used to develop a method for the rational preparation of molecularly imprinted polymers (MIPs) for protein recognition. The fingerprint imprinting (FIP) is based on the following: (1) the in silico cleavage of the protein sequence of interest with specific agents; (2) the screening of all the peptide sequences generated against the UniProtKB database in order to allow for the rational selection of distinctive and unique peptides (named as epitopes) of the target protein; (3) the selected epitopes are synthesized and used as templates for the molecular imprinting process. To prove the principle, NT-proBNP, a marker of the risk of cardiovascular events, was chosen as an example. The in silico analysis of the NT-proBNP sequence allowed us to individuate the peptide candidates, which were next used as templates for the preparation of NT-pro-BNP-specific FIPs and tested for their ability to bind the NT-proBNP peptides in complex samples. Results indicated an imprinting factor, IF, of ~10, a binding capacity of 0.5-2 mg/g, and the ability to rebind 40% of the template in a complex sample, composed of the whole digests of NT-proBNP.
- Subjects :
- Amino Acid Sequence
Cardiovascular Diseases diagnosis
Humans
Natriuretic Peptide, Brain isolation & purification
Natriuretic Peptide, Brain metabolism
Peptide Fragments isolation & purification
Peptide Fragments metabolism
Peptides metabolism
Polymers metabolism
Protein Binding
Molecular Imprinting methods
Natriuretic Peptide, Brain blood
Peptide Fragments blood
Peptides chemistry
Polymers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 84
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22462546
- Full Text :
- https://doi.org/10.1021/ac203422r