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A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2012 May 15; Vol. 35 (1), pp. 291-296. Date of Electronic Publication: 2012 Mar 10. - Publication Year :
- 2012
-
Abstract
- Finding a highly sensitive diagnostic technique for malaria has challenged scientists for the last century. In the present study, we identified versatile single-strand DNA aptamers for Plasmodium lactate dehydrogenase (pLDH), a biomarker for malaria, via the Systematic Evolution of Ligands by EXponential enrichment (SELEX). The pLDH aptamers selectively bound to the target proteins with high sensitivity (K(d)=16.8-49.6 nM). The selected aptamers were characterized using an electrophoretic mobility shift assay, a quartz crystal microbalance, a fluorescence assay, and circular dichroism spectroscopy. We also designed a simple aptasensor using electrochemical impedance spectroscopy; both Plasmodium vivax LDH and Plasmodium falciparum LDH were selectively detected with a detection limit of 1 pM. Furthermore, the pLDH aptasensor clearly distinguished between malaria-positive blood samples of two major species (P. vivax and P. falciparum) and a negative control, indicating that it may be a useful tool for the diagnosis, monitoring, and surveillance of malaria.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Base Sequence
Biomarkers blood
Biosensing Techniques statistics & numerical data
Circular Dichroism
Dielectric Spectroscopy
Electrophoretic Mobility Shift Assay
Humans
Limit of Detection
Malaria enzymology
Malaria parasitology
Malaria, Falciparum diagnosis
Malaria, Vivax diagnosis
Nucleic Acid Conformation
Quartz Crystal Microbalance Techniques
SELEX Aptamer Technique statistics & numerical data
Aptamers, Nucleotide chemistry
Biosensing Techniques methods
L-Lactate Dehydrogenase blood
Malaria diagnosis
Plasmodium enzymology
SELEX Aptamer Technique methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 22459583
- Full Text :
- https://doi.org/10.1016/j.bios.2012.03.003