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The effect of allogenetic bone marrow-derived mesenchymal stem cell transplantation on lung aquaporin-1 and -5 in a rat model of severe acute pancreatitis.
- Source :
-
Hepato-gastroenterology [Hepatogastroenterology] 2012 Jun; Vol. 59 (116), pp. 965-76. - Publication Year :
- 2012
-
Abstract
- Background/aims: We aimed to determine the effect of transplantation on post- ALI (acute lung injury) edema in severe acute pancreatitis (SAP) and the expression levels of aquaporins -1 and -5 (AQP-1 and -5).<br />Methodology: Sprague-Dawley (SD) rats were randomized into control-SAP and BMSCs-SAP groups. SAP model was prepared through retrograde injection of 5% taurocholic acid. BMSCs were isolated from the bone marrow of SD rats. We examined SAP rats for levels of IL-1β and TNF-a, and for AQP-1 and -5 expression in lung tissues at 6 and 12 hours.<br />Results: The levels of IL-1β and TNF-a in BMSC-SAP rats were lower than in control-SAP rats (both, p<0.001). Real-time RT-PCR analysis showed that AQP-1 mRNA expression in BMSC-SAP rats was higher than that in control-SAP rats (p=0.005 and p<0.001), and AQP-5 mRNA expression in BMSC-SAP rats was also higher than that in control-SAP rats (p=0.031 and p=0.006). Western blotting analysis showed that AQP-1 and AQP-5 protein levels at 12h were significantly higher in BMSC-SAP rats than in control-SAP rats (p<0.001).<br />Conclusions: Allogenic BMSC transplantation can protect against ALI in a rat SAP model and can also regulate the expression levels of AQP-1 and -5 by inhibiting IL-1β and TNF-a.
- Subjects :
- Acute Disease
Amylases blood
Animals
Aquaporin 1 genetics
Aquaporin 5 genetics
Cells, Cultured
Disease Models, Animal
Lung metabolism
Lung pathology
Male
Mesenchymal Stem Cell Transplantation
Organ Size
Pancreatitis metabolism
Permeability
Rats
Rats, Sprague-Dawley
Serum Albumin analysis
Transplantation, Homologous
Aquaporin 1 analysis
Aquaporin 5 analysis
Lung chemistry
Pancreatitis therapy
Subjects
Details
- Language :
- English
- ISSN :
- 0172-6390
- Volume :
- 59
- Issue :
- 116
- Database :
- MEDLINE
- Journal :
- Hepato-gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 22456279
- Full Text :
- https://doi.org/10.5754/hge12094