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Confirmation of drug delivery after liver chemoembolization: direct tissue doxorubicin measurement by UHPLC-MS-MS.
- Source :
-
Biomedical chromatography : BMC [Biomed Chromatogr] 2012 Dec; Vol. 26 (12), pp. 1529-33. Date of Electronic Publication: 2012 Mar 27. - Publication Year :
- 2012
-
Abstract
- Because liver cancer is rarely suitable for surgery, transcatheter arterial chemoembolization (TACE) is used for palliative therapy. In this procedure, an emulsion of doxorubicin in iodized oil is injected directly into liver tumors through a catheter positioned within the artery supplying blood flow to the tumor. At present, there is limited understanding of factors affecting the delivery and dispersion of doxorubicin within treated tumors during TACE. This study addresses the development and application of an ultrahigh-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS-MS) method for rapid confirmation of drug delivery after TACE in a rabbit VX2 liver cancer model. Doxorubicin levels in liver tumors were measured using UHPLC-MS-MS and compared with computed tomography measured levels of iodized oil, a metric used clinically to indicate drug delivery. We found that tissue drug levels determined using UHPLC-MS-MS did not correlate with the regional iodized oil concentration (vehicle) within tumors following TACE, suggesting that chemotherapeutic drugs like doxorubicin spread throughout tumors, and that lack of iodized oil staining in portions of a tumor does not necessarily indicate inadequate therapy during TACE.<br /> (Copyright © 2012 John Wiley & Sons, Ltd.)
- Subjects :
- Animals
Antibiotics, Antineoplastic administration & dosage
Antibiotics, Antineoplastic analysis
Antibiotics, Antineoplastic pharmacokinetics
Chemoembolization, Therapeutic instrumentation
Doxorubicin administration & dosage
Doxorubicin pharmacokinetics
Drug Delivery Systems
Emulsions chemistry
Ethiodized Oil pharmacokinetics
Liver Neoplasms, Experimental metabolism
Liver Neoplasms, Experimental pathology
Male
Rabbits
Tomography, X-Ray Computed
Chemoembolization, Therapeutic methods
Chromatography, High Pressure Liquid methods
Doxorubicin analysis
Liver Neoplasms, Experimental chemistry
Liver Neoplasms, Experimental therapy
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0801
- Volume :
- 26
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Biomedical chromatography : BMC
- Publication Type :
- Academic Journal
- Accession number :
- 22454282
- Full Text :
- https://doi.org/10.1002/bmc.2727