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Maturation of periodontal tissues following implantation of rhGDF-5/β-TCP in one-wall intra-bony defects in dogs: 24-week histological observations.
- Source :
-
Journal of clinical periodontology [J Clin Periodontol] 2012 May; Vol. 39 (5), pp. 466-74. Date of Electronic Publication: 2012 Mar 26. - Publication Year :
- 2012
-
Abstract
- Objective: Although a previous study reported that recombinant human growth/differentiation factor-5 (rhGDF-5) coated onto a β-tricalciumphosphate (β-TCP) significantly enhanced periodontal regeneration, the long-term stability/maturation of the regenerated tissues has not been demonstrated. The objective of this study was to evaluate periodontal regeneration/maturation following application of rhGDF-5/β-TCP using an established periodontal defect model and a 24-week healing interval.<br />Material & Methods: Unilateral, surgically created, 4 × 4 × 5 mm (length × width × height), one-wall, critical-size, intra-bony periodontal defects at the mandibular second and fourth premolar teeth in five young adult Beagle dogs received rhGDF-5/β-TCP. Bilateral sites at the fourth premolar in the other four dogs served as pristine controls receiving mucogingival flap surgery without defect induction. The animals were euthanized at 24 weeks for histological analysis. Unpublished data from the previous 8-week study were used to compare tissue maturation between 8 and 24 weeks.<br />Results: Linear histometric observations of cementum and alveolar regeneration showed no significant differences between the 8- and 24-week observation intervals. However, parameters of periodontal tissue maturation showed significant differences between the observation intervals including increased fraction mineralized tissue and lamellar bone (p < 0.05) and decreased osteocyte counts (p < 0.05) at 24 weeks compared with 8 weeks. Although the count inserting Sharpey's fibre did not significantly change, regenerated cementum remote from the intact periodontal ligament appeared more highly mineralized and thicker at 24 weeks compared with 8 weeks, and compared with the pristine cementum. Minimal β-TCP remained.<br />Conclusions: These 24-week observations suggest that regenerated periodontal tissues in sites receiving rhGDF-5/β-TCP undergo progressive maturation without debilitating aberrant tissue reactions.<br /> (© 2012 John Wiley & Sons A/S.)
- Subjects :
- Absorbable Implants
Alveolar Bone Loss pathology
Alveolar Process drug effects
Alveolar Process pathology
Animals
Bone Regeneration drug effects
Calcification, Physiologic physiology
Cell Count
Collagen drug effects
Dental Cementum drug effects
Dental Cementum pathology
Dogs
Epithelial Attachment drug effects
Epithelial Attachment pathology
Humans
Male
Mandible drug effects
Mandible pathology
Osteocytes drug effects
Osteocytes pathology
Periodontal Ligament drug effects
Periodontal Ligament pathology
Random Allocation
Recombinant Proteins
Time Factors
Tissue Engineering methods
Alveolar Bone Loss surgery
Biocompatible Materials
Calcium Phosphates
Growth Differentiation Factor 5 therapeutic use
Guided Tissue Regeneration, Periodontal methods
Tissue Scaffolds
Subjects
Details
- Language :
- English
- ISSN :
- 1600-051X
- Volume :
- 39
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of clinical periodontology
- Publication Type :
- Academic Journal
- Accession number :
- 22449372
- Full Text :
- https://doi.org/10.1111/j.1600-051X.2012.01862.x