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Two novel N-terminal coding exons of Prkar1b gene of mouse: identified using a novel approach of in silico and molecular biology techniques.
- Source :
-
Gene [Gene] 2012 May 25; Vol. 500 (1), pp. 73-9. Date of Electronic Publication: 2012 Mar 16. - Publication Year :
- 2012
-
Abstract
- The Prkar1b gene encodes regulatory type 1 beta subunit of protein kinase A. Here we report that mouse R1β gene produces three alternative splice variants (designated as mR1β1, mR1β2 and mR1β3) that have different N-terminal protein structures. New splice variants were identified using combinatorial approach of bioinformatics pipeline involving online available tools and databases, and molecular biology techniques involving RT-PCR, semi-nested PCR and sequencing. Except mR1β3, which was not detected by RT-PCR in brain and muscle tissues of 3day old mice, all three spliced isoforms were found to be ubiquitously expressed in tissues and postnatal developmental stages examined. The presence of different N-termini in isoforms may be important for unique docking interactions with A kinase anchoring proteins.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Exons
Mice
Molecular Sequence Data
Organ Specificity
Protein Isoforms chemistry
Protein Isoforms genetics
Sequence Alignment
Alternative Splicing
Cyclic AMP-Dependent Protein Kinase RIbeta Subunit chemistry
Cyclic AMP-Dependent Protein Kinase RIbeta Subunit genetics
Molecular Biology methods
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0038
- Volume :
- 500
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 22446042
- Full Text :
- https://doi.org/10.1016/j.gene.2012.02.051