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Streptococcus pneumoniae-induced regulation of cyclooxygenase-2 in human lung tissue.
- Source :
-
The European respiratory journal [Eur Respir J] 2012 Dec; Vol. 40 (6), pp. 1458-67. Date of Electronic Publication: 2012 Mar 22. - Publication Year :
- 2012
-
Abstract
- The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue. Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites. In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid(4) receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies, cyclooxygenase-2/prostaglandin E(2) related pro- and anti-inflammatory mediator regulation was partly confirmed in human lung tissue after pneumococcal infection. Overall, cell type-specific and MAPK-dependent cyclooxygenase-2 expression and prostaglandin E(2) formation in human lung tissue may play an important role in the early phase of pneumococcal infections.
- Subjects :
- Colony-Forming Units Assay
Dinoprostone metabolism
Epithelial Cells microbiology
Extracellular Signal-Regulated MAP Kinases metabolism
Gene Expression Profiling
Humans
Immunohistochemistry methods
Inflammation
MAP Kinase Signaling System
Microscopy, Fluorescence methods
Pneumococcal Infections enzymology
Prostaglandins metabolism
Pulmonary Alveoli microbiology
Cyclooxygenase 2 metabolism
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Lung enzymology
Lung microbiology
Pneumococcal Infections microbiology
Streptococcus pneumoniae metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1399-3003
- Volume :
- 40
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The European respiratory journal
- Publication Type :
- Academic Journal
- Accession number :
- 22441740
- Full Text :
- https://doi.org/10.1183/09031936.00186911