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Green biosynthesis of floxuridine by immobilized microorganisms.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2012 Jun; Vol. 331 (1), pp. 31-6. Date of Electronic Publication: 2012 Apr 04. - Publication Year :
- 2012
-
Abstract
- This work describes an efficient, simple, and green bioprocess for obtaining 5-halogenated pyrimidine nucleosides from thymidine by transglycosylation using whole cells. Biosynthesis of 5-fluoro-2'-deoxyuridine (floxuridine) was achieved by free and immobilized Aeromonas salmonicida ATCC 27013 with an 80% and 65% conversion occurring in 1 h, respectively. The immobilized biocatalyst was stable for more than 4 months in storage conditions (4 °C) and could be reused at least 30 times without loss of its activity. This microorganism was able to biosynthesize 2.0 mg L(-1) min(-1) (60%) of 5-chloro-2'-deoxyuridine in 3 h. These halogenated pyrimidine 2'-deoxynucleosides are used as antitumoral agents.<br /> (© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1574-6968
- Volume :
- 331
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 22428623
- Full Text :
- https://doi.org/10.1111/j.1574-6968.2012.02547.x