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Tissue factor/factor VIIa complex: role of the membrane surface.

Authors :
Morrissey JH
Tajkhorshid E
Sligar SG
Rienstra CM
Source :
Thrombosis research [Thromb Res] 2012 May; Vol. 129 Suppl 2, pp. S8-10. Date of Electronic Publication: 2012 Mar 12.
Publication Year :
2012

Abstract

Blood clotting is triggered when the plasma serine protease factor VIIa binds to the cell-surface protein, tissue factor (TF); the resulting TF:FVIIa complex activates factors IX (FIX) and X (FX) by limited proteolysis. FVIIa, FIX and FX all bind reversibly to membranes via their gamma-carboxyglutamate-rich (GLA) domains, while TF is an integral membrane protein. Removing these proteases from the membrane surface is known to render them thousands of times less active, although the mechanisms by which blood clotting proteins bind to membranes-and the contributions of membranes to catalysis-remain very incompletely understood. Our recent and ongoing studies use a combination of nanoscale membrane bilayers (Nanodiscs), solid-state NMR and all-atom molecular dynamics (MD) simulations, enabling detailed insights into how GLA domains bind to phospholipid bilayers and how specific phospholipids enhance the catalytic activity of the TF:FVIIa complex.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1879-2472
Volume :
129 Suppl 2
Database :
MEDLINE
Journal :
Thrombosis research
Publication Type :
Academic Journal
Accession number :
22417943
Full Text :
https://doi.org/10.1016/j.thromres.2012.02.019