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Corneal and retinal effects of ultraviolet-B exposure in a soft contact lens mouse model.

Authors :
Ibrahim OM
Kojima T
Wakamatsu TH
Dogru M
Matsumoto Y
Ogawa Y
Ogawa J
Negishi K
Shimazaki J
Sakamoto Y
Sasaki H
Tsubota K
Source :
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2012 Apr 30; Vol. 53 (4), pp. 2403-13. Date of Electronic Publication: 2012 Apr 30.
Publication Year :
2012

Abstract

Purpose: To investigate the lipid and DNA oxidative stress as well as corneal and retinal effects after ultraviolet B (UV-B) exposure in mice, with or without silicon hydrogel soft contact lenses (SCL).<br />Methods: Twenty-eight C57BL6-strain male mice were divided into four groups: group I, control group with no SCL (SCL [-]) and no UV-B exposure (UV-B [-]); group II, senofilcon A SCL (senofilcon [+]) with UV-B exposure (UV-B [+]); group III, lotrafilcon A SCL (lotrafilcon [+]) with UV-B exposure (UV-B [+]); and group IV, no SCL (SCL [-]), but with UV-B exposure (UV-B [+]). All mice except group I received UV-B exposure for 5 days for a total dose of 2.73 J/cm(2). All mice underwent tear hexanoyl-lysine (HEL) and tear cytokine ELISA measurements, and fluorescein and rose bengal corneal staining before and after UV-B exposure. Corneal specimens underwent immunohistochemistry staining with CD45, HEL, 4-hydroxynonenal (4-HNE), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) antibodies and evaluation with electron microscopy.<br />Results: All mice without SCL but exposed to UV-B developed corneal edema, ulcers, or epithelial damage compared with mice with senofilcon A SCL and exposure to UV-B. Tear HEL and cytokine levels significantly increased in mice without SCL after UV-B exposure. Immunohistochemistry showed a significantly higher number of cells positively stained for CD45, 8-OHdG, HEL, and 4-HNE in the corneas of mice without SCLs compared with those with senofilcon A after UV-B exposure.<br />Conclusion: Silicon hydrogel SCL showed corneal and retinal protective effects, owing to UV blocking properties, against oxidative stress-related membrane lipid and cellular DNA damage.

Details

Language :
English
ISSN :
1552-5783
Volume :
53
Issue :
4
Database :
MEDLINE
Journal :
Investigative ophthalmology & visual science
Publication Type :
Academic Journal
Accession number :
22410564
Full Text :
https://doi.org/10.1167/iovs.11-6863