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Corneal and retinal effects of ultraviolet-B exposure in a soft contact lens mouse model.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2012 Apr 30; Vol. 53 (4), pp. 2403-13. Date of Electronic Publication: 2012 Apr 30. - Publication Year :
- 2012
-
Abstract
- Purpose: To investigate the lipid and DNA oxidative stress as well as corneal and retinal effects after ultraviolet B (UV-B) exposure in mice, with or without silicon hydrogel soft contact lenses (SCL).<br />Methods: Twenty-eight C57BL6-strain male mice were divided into four groups: group I, control group with no SCL (SCL [-]) and no UV-B exposure (UV-B [-]); group II, senofilcon A SCL (senofilcon [+]) with UV-B exposure (UV-B [+]); group III, lotrafilcon A SCL (lotrafilcon [+]) with UV-B exposure (UV-B [+]); and group IV, no SCL (SCL [-]), but with UV-B exposure (UV-B [+]). All mice except group I received UV-B exposure for 5 days for a total dose of 2.73 J/cm(2). All mice underwent tear hexanoyl-lysine (HEL) and tear cytokine ELISA measurements, and fluorescein and rose bengal corneal staining before and after UV-B exposure. Corneal specimens underwent immunohistochemistry staining with CD45, HEL, 4-hydroxynonenal (4-HNE), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) antibodies and evaluation with electron microscopy.<br />Results: All mice without SCL but exposed to UV-B developed corneal edema, ulcers, or epithelial damage compared with mice with senofilcon A SCL and exposure to UV-B. Tear HEL and cytokine levels significantly increased in mice without SCL after UV-B exposure. Immunohistochemistry showed a significantly higher number of cells positively stained for CD45, 8-OHdG, HEL, and 4-HNE in the corneas of mice without SCLs compared with those with senofilcon A after UV-B exposure.<br />Conclusion: Silicon hydrogel SCL showed corneal and retinal protective effects, owing to UV blocking properties, against oxidative stress-related membrane lipid and cellular DNA damage.
- Subjects :
- Animals
Case-Control Studies
Cornea pathology
Cytokines metabolism
Cytokines radiation effects
DNA Damage
Enzyme-Linked Immunosorbent Assay
Lipid Metabolism
Lipids radiation effects
Lysine metabolism
Lysine radiation effects
Male
Mice
Mice, Inbred C57BL
Oxidative Stress
Retina pathology
Silicones therapeutic use
Tears chemistry
Contact Lenses, Hydrophilic
Cornea radiation effects
Hydrogels therapeutic use
Radiation Injuries, Experimental prevention & control
Retina radiation effects
Ultraviolet Rays adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5783
- Volume :
- 53
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 22410564
- Full Text :
- https://doi.org/10.1167/iovs.11-6863