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Increased halogenated tyrosine levels are useful markers of human skin ageing, reflecting proteins denatured by past skin inflammation.
- Source :
-
Clinical and experimental dermatology [Clin Exp Dermatol] 2012 Apr; Vol. 37 (3), pp. 252-8. - Publication Year :
- 2012
-
Abstract
- Background: Photoageing of skin is thought to be caused by protein denaturation, which can be induced by ultraviolet radiation. Previous studies have also reported that inflammation is related to protein denaturation; however, the influence of inflammation on skin ageing has not been explored in detail.<br />Aim: To investigate the possible connection between inflammation and protein denaturation, which might lead to skin ageing, we focused on halogenated tyrosine as a denatured substance produced during the inflammation process.<br />Methods: We measured halogenated tyrosine in aged human skin. Inflammatory cells and halogenated tyrosine were detected by immunohistochemistry using antibodies to mast-cell tryptase, neutrophilic myeloperoxidase and halogenated tyrosine. Finally, using elastic van Gieson (EVG) staining, we investigated whether the sites of halogenated tyrosine coincided with the sites at which proteins were denatured.<br />Results: Immunohistochemical analysis indicated that both inflammatory cells and halogenated tyrosines increased with ageing in both photoexposed and photoprotected skin. EVG staining confirmed that the localization of halogenated tyrosine was close to the sites at which protein was denatured.<br />Conclusions: Our investigations indicate a possible connection between skin ageing and inflammation, suggesting that halogenated tyrosine could be a useful marker of ageing skin.<br /> (© The Author(s). CED © 2012 British Association of Dermatologists.)
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Biomarkers metabolism
Child
Female
Halogenation
Humans
Immunohistochemistry
Male
Mast Cells cytology
Middle Aged
Neutrophils cytology
Skin cytology
Tyrosine radiation effects
Young Adult
Inflammation metabolism
Protein Denaturation
Skin radiation effects
Skin Aging
Tyrosine metabolism
Ultraviolet Rays adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2230
- Volume :
- 37
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Clinical and experimental dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 22409522
- Full Text :
- https://doi.org/10.1111/j.1365-2230.2011.04215.x