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Biofilm formation on nanostructured titanium oxide surfaces and a micro/nanofabrication-based preventive strategy using colloidal lithography.
- Source :
-
Biofabrication [Biofabrication] 2012 Jun; Vol. 4 (2), pp. 025001. Date of Electronic Publication: 2012 Mar 12. - Publication Year :
- 2012
-
Abstract
- The contamination of implant devices as a result of biofilm formation through bacterial infection has instigated major research in this area, particularly to understand the mechanism of bacterial cell/implant surface interactions and their preventions. In this paper, we demonstrate a controlled method of nanostructured titanium oxide surface synthesis using supersonic cluster beam depositions. The nanoscale surface characterization using atomic force microscopy and a profilometer display a regulated evolution in nanomorphology and physical properties. X-ray photoelectron spectroscopy analyses display a stoichiometric nanostructured TiO(2) film. Measurement of the water contact angle shows a nominal increase in the hydrophilic nature of ns-TiO(2) films, whereas the surface energy increases with decreasing contact angle. Bacterial species Staphylococcus aureus and Escherichia coli interaction with nanostructured surfaces shows an increase in adhesion and biofilm formation with increasing nanoscale morphological properties. Conversely, limiting ns-TiO(2) film distribution to micro/nanopatterned designed substrates integrated with bovine serum albumin functionalization leads to a reduction in biofilm formations due to a globally decreased bacterial cell-surface interaction area. The results have potential implications in inhibiting bacterial colonization and promoting mammalian cell-implant interactions.
- Subjects :
- Animals
Cattle
Escherichia coli drug effects
Escherichia coli physiology
Microscopy
Nanostructures ultrastructure
Printing
Serum Albumin, Bovine chemistry
Staphylococcus aureus drug effects
Staphylococcus aureus physiology
Surface Properties
Titanium chemistry
Bacterial Adhesion drug effects
Biofilms drug effects
Biofilms growth & development
Nanostructures chemistry
Nanotechnology methods
Titanium pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1758-5090
- Volume :
- 4
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biofabrication
- Publication Type :
- Academic Journal
- Accession number :
- 22406493
- Full Text :
- https://doi.org/10.1088/1758-5082/4/2/025001