Back to Search
Start Over
Membrane-displayed peptide ligand activates the pheromone response pathway in Saccharomyces cerevisiae.
- Source :
-
Journal of biochemistry [J Biochem] 2012 May; Vol. 151 (5), pp. 551-7. Date of Electronic Publication: 2012 Mar 08. - Publication Year :
- 2012
-
Abstract
- The budding yeast, Saccharomyces cerevisiae, is an attractive host for studying G protein-coupled receptors (GPCRs). We developed a system in which a peptide ligand specific for GPCR is displayed on yeast plasma membrane. The model system described here is based on yeast plasma membrane display of an analogue of α-factor, which is a peptide ligand for Ste2p, the GPCR that activates the yeast pheromone response pathway. α-Factor analogues, containing linkers of varying lengths and produced in yeast cells, became attached to the cell plasma membrane by linking to the glycosylphosphatidylinositol (GPI)-anchored plasma membrane protein Yps1p. We were able to demonstrate that an optimized α-factor analogue activated the pheromone response pathway in S. cerevisiae, as assessed by a fluorescent reporter assay. Furthermore, it was shown that linker length strongly influenced signalling pathway activation. To our knowledge, this is the first report documenting functional signalling by a plasma membrane-displayed ligand in S. cerevisiae.
- Subjects :
- Cell Membrane chemistry
Ligands
Peptides chemistry
Peptides metabolism
Receptors, G-Protein-Coupled antagonists & inhibitors
Receptors, G-Protein-Coupled metabolism
Saccharomyces cerevisiae cytology
Cell Membrane metabolism
Peptides pharmacology
Pheromones pharmacology
Saccharomyces cerevisiae drug effects
Saccharomyces cerevisiae metabolism
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1756-2651
- Volume :
- 151
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22406406
- Full Text :
- https://doi.org/10.1093/jb/mvs027