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Prevalence of latent alpha-herpesviruses in Thoroughbred racing horses.
- Source :
-
Veterinary journal (London, England : 1997) [Vet J] 2012 Aug; Vol. 193 (2), pp. 579-82. Date of Electronic Publication: 2012 Mar 10. - Publication Year :
- 2012
-
Abstract
- The objective of this study was to detect and characterize latent equine herpes virus (EHV)-1 and -4 from the submandibular (SMLN) and bronchial lymph (BLN) nodes, as well as from the trigeminal ganglia (TG) of 70 racing Thoroughbred horses submitted for necropsy following sustaining serious musculoskeletal injuries while racing. A combination of nucleic acid precipitation and pre-amplification steps was used to increase analytical sensitivity. Tissues were deemed positive for latent EHV-1 and/or -4 infection when found PCR positive for the corresponding glycoprotein B (gB) gene in the absence of detectable late structural protein gene (gB gene) mRNA. The EHV-1 genotype was also determined using a discriminatory real-time PCR assay targeting the DNA polymerase gene (ORF 30). Eighteen (25.7%) and 58 (82.8%) horses were PCR positive for the gB gene of EHV-1 and -4, respectively, in at least one of the three tissues sampled. Twelve horses were dually infected with EHV-1 and -4, two carried a latent neurotropic strain of EHV-1, six carried a non-neurotropic genotype of EHV-1 and 10 were dually infected with neurotropic and non-neurotropic EHV-1. The distribution of latent EHV-1 and -4 infection varied in the samples, with the TG found to be most commonly infected. Overall, non-neurotropic strains were more frequently detected than neurotropic strains, supporting the general consensus that non-neurotropic strains are more prevalent in horse populations, and hence the uncommon occurrence of equine herpes myeloencephalopathy.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bronchi virology
California epidemiology
DNA, Viral genetics
Female
Herpesviridae Infections epidemiology
Herpesviridae Infections virology
Herpesvirus 1, Equid isolation & purification
Herpesvirus 4, Equid isolation & purification
Horse Diseases virology
Horses
Male
Mandible virology
Pedigree
Prevalence
Real-Time Polymerase Chain Reaction veterinary
Trigeminal Ganglion virology
Herpesviridae Infections veterinary
Herpesvirus 1, Equid physiology
Herpesvirus 4, Equid physiology
Horse Diseases epidemiology
Lymph Nodes virology
Virus Latency
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2971
- Volume :
- 193
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Veterinary journal (London, England : 1997)
- Publication Type :
- Academic Journal
- Accession number :
- 22405721
- Full Text :
- https://doi.org/10.1016/j.tvjl.2012.01.030