Back to Search
Start Over
A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay.
- Source :
-
Gene therapy [Gene Ther] 2013 Feb; Vol. 20 (2), pp. 169-76. Date of Electronic Publication: 2012 Mar 08. - Publication Year :
- 2013
-
Abstract
- Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S(+)L(-) or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S(+)L(-) assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing.
Details
- Language :
- English
- ISSN :
- 1476-5462
- Volume :
- 20
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Gene therapy
- Publication Type :
- Academic Journal
- Accession number :
- 22402321
- Full Text :
- https://doi.org/10.1038/gt.2012.18