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Role of serotonergic system in the pathogenesis of fibrosis in canine idiopathic inflammatory myopathies.
- Source :
-
Neuromuscular disorders : NMD [Neuromuscul Disord] 2012 Jun; Vol. 22 (6), pp. 549-57. Date of Electronic Publication: 2012 Mar 06. - Publication Year :
- 2012
-
Abstract
- Idiopathic inflammatory myopathies are muscle diseases characterized by inflammation, necrosis, and fibrosis. The neurotransmitter serotonin (5-HT) has been shown to promote fibrosis in many tissues and organs by activating TGFβ-1 signaling. In this study, we evaluated the potential role of 5-HT in the pathogenesis of fibrosis in canine idiopathic inflammatory myopathies. Muscle biopsies from dogs affected by masticatory muscle myositis or polymyositis and from healthy dogs were processed for immunohistochemistry and Western blotting. The immunohistochemical analysis showed a strong expression of 5-HT in muscle tissues of affected dogs, whereas the amine was absent in the muscles of healthy dogs. Biochemical analysis showed increased expression levels of the selective 5-HT2A receptor in the muscle specimens of the most severely affected dogs versus controls. Further, increased phosphorylation levels of the TGFβ-1 signaling mediators SMAD2/3 and ERK1/2 were detected in tissue samples from affected dogs as compared to tissues from healthy dogs. Although further studies are needed, our findings highlight for the first time a potential role of 5-HT in the development of fibrosis in canine idiopathic inflammatory myopathies, thus supporting other evidence that 5-HT pro-fibrotic activity occurs via activation of TGFβ-1 signaling pathway.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Dog Diseases pathology
Dogs
Female
Fibrosis metabolism
Fibrosis pathology
Male
Muscle, Skeletal pathology
Myositis metabolism
Myositis pathology
Phosphorylation
Receptor, Serotonin, 5-HT2A metabolism
Signal Transduction physiology
Dog Diseases metabolism
Fibrosis veterinary
Muscle, Skeletal metabolism
Myositis veterinary
Serotonin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2364
- Volume :
- 22
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Neuromuscular disorders : NMD
- Publication Type :
- Academic Journal
- Accession number :
- 22397866
- Full Text :
- https://doi.org/10.1016/j.nmd.2012.01.009