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Expression of podoplanin in human astrocytic brain tumors is controlled by the PI3K-AKT-AP-1 signaling pathway and promoter methylation.
- Source :
-
Neuro-oncology [Neuro Oncol] 2012 Apr; Vol. 14 (4), pp. 426-39. Date of Electronic Publication: 2012 Mar 06. - Publication Year :
- 2012
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Abstract
- Recently, we found strong overexpression of the mucin-type glycoprotein podoplanin (PDPN) in human astrocytic brain tumors, specifically in primary glioblastoma multiforme (GB). In the current study, we show an inverse correlation between PDPN expression and PTEN levels in primary human GB and glioma cell lines, and we report elevated PDPN protein levels in the subventricular zone of brain tissue sections of PTEN-deficient mice. In human glioma cells lacking functional PTEN, reintroduction of wild-type PTEN, inhibition of the PTEN downstream target protein kinase B/AKT, or interference with transcription factor AP-1 function resulted in efficient downregulation of PDPN expression. In addition, we observed hypoxia-dependent PDPN transcriptional control and demonstrated that PDPN expression is subject to negative transcriptional regulation by promoter methylation in human GB and in glioma cell lines. Treatment of PTEN-negative glioma cells with demethylating agents induced expression of PDPN. Together, our findings show that increased PDPN expression in human GB is caused by loss of PTEN function and activation of the PI3K-AKT-AP-1 signaling pathway, accompanied by epigenetic regulation of PDPN promoter activity. Silencing of PDPN expression leads to reduced proliferation and migration of glioma cells, suggesting a functional role of PDPN in glioma progression and malignancy. Thus, specific targeting of PDPN expression and/or function could be a promising strategy for the treatment of patients with primary GB.
- Subjects :
- Animals
Astrocytoma genetics
Brain Neoplasms genetics
DNA Methylation
Down-Regulation
Glioblastoma genetics
Glioma genetics
Humans
Membrane Glycoproteins genetics
Mice
PTEN Phosphohydrolase genetics
PTEN Phosphohydrolase physiology
Phosphatidylinositol 3-Kinases genetics
Phosphatidylinositol 3-Kinases metabolism
Promoter Regions, Genetic
Proto-Oncogene Proteins c-akt genetics
Proto-Oncogene Proteins c-akt metabolism
Signal Transduction
Transcription Factor AP-1 genetics
Transcription Factor AP-1 metabolism
Astrocytoma metabolism
Brain Neoplasms metabolism
Gene Expression Regulation, Neoplastic
Glioblastoma metabolism
Glioma metabolism
Membrane Glycoproteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1523-5866
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Neuro-oncology
- Publication Type :
- Academic Journal
- Accession number :
- 22394497
- Full Text :
- https://doi.org/10.1093/neuonc/nos055