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Ciprofloxacin and epirubicin synergistically induce apoptosis in human urothelial cancer cell lines.
- Source :
-
Urologia internationalis [Urol Int] 2012; Vol. 88 (3), pp. 343-9. Date of Electronic Publication: 2012 Mar 01. - Publication Year :
- 2012
-
Abstract
- Introduction: Few published in vitro studies have shown antitumor drug action or possible synergistic effects of fluoroquinolones. To assess the potential role of combination therapy, cytotoxic effects of ciprofloxacin and epirubicin alone and in combination were determined.<br />Material and Methods: Human urothelial cancer cell lines HT1197 and HT1376 were exposed in vitro for 1 h to different concentrations of epirubicin (0.02-2 mg/ml) and for 72 h to ciprofloxacin (0.004-0.8 mg/ml). Cytotoxicity was determined using the microculture tetrazolium assay and flow cytometry. Synergistic cytotoxic effects were determined by calculating combination indices.<br />Results: Median effect concentrations of epirubicin for HT1376 and HT1197 cells were as low as 124 and 117 μg/ml, respectively. Ciprofloxacin-treated cells exhibited profound cytotoxic effects at concentrations of 50-100 μg/ml, which is far below the intravesical concentration reached by standard oral application. In addition, a pronounced synergistic effect was found when the two treatments were combined.<br />Conclusions: This study provides evidence that ciprofloxacin and epirubicin exhibit synergistic cytotoxic effects in vitro. After confirmatory animal experiments, future clinical studies of adjuvant chemotherapy after transurethral bladder resection may include treatment arms with combinations of fluoroquinolones based on the observed synergistic effects to reduce both side effects and costs.<br /> (Copyright © 2012 S. Karger AG, Basel.)
- Subjects :
- Adult
Cell Line, Tumor
Ciprofloxacin pharmacology
Dose-Response Relationship, Drug
Drug Synergism
Epirubicin pharmacology
Female
Flow Cytometry
Humans
Male
Middle Aged
Time Factors
Urothelium pathology
Antineoplastic Combined Chemotherapy Protocols pharmacology
Apoptosis drug effects
Urologic Neoplasms pathology
Urothelium drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0399
- Volume :
- 88
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Urologia internationalis
- Publication Type :
- Academic Journal
- Accession number :
- 22378292
- Full Text :
- https://doi.org/10.1159/000336130