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Preparation and characterization of biocompatible chitosan nanoparticles for targeted brain delivery of peptides.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 846, pp. 321-32. - Publication Year :
- 2012
-
Abstract
- Here, we describe a nanocarrier system that can transfer chitosan nanoparticles loaded with either small peptides such as the caspase inhibitor Z-DEVD-FMK or a large peptide like basic fibroblast growth factor across the blood-brain barrier. The nanoparticles are selectively directed to the brain and are not measurably taken up by liver and spleen. Intravital fluorescent microscopy provides an opportunity to study the penetration kinetics of nanoparticles loaded with fluorescent agents such as Nile red, and has demonstrated that this nanomedicine formulation is rapidly transported across the blood-brain barrier.
- Subjects :
- Antibodies, Monoclonal
Blood-Brain Barrier metabolism
Fibroblast Growth Factors
Kinetics
Microscopy, Electron, Scanning
Molecular Structure
Oligopeptides
Peptides administration & dosage
Streptavidin
Biocompatible Materials chemistry
Chitosan chemistry
Drug Delivery Systems methods
Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 846
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 22367822
- Full Text :
- https://doi.org/10.1007/978-1-61779-536-7_27