Back to Search
Start Over
Automated zebrafish chorion removal and single embryo placement: optimizing throughput of zebrafish developmental toxicity screens.
- Source :
-
Journal of laboratory automation [J Lab Autom] 2012 Feb; Vol. 17 (1), pp. 66-74. - Publication Year :
- 2012
-
Abstract
- The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating and chemically exposing zebrafish embryos-namely, manual placement of embryos into 96- or 384-well plates and exposure of embryos while still in the chorion, a barrier of poorly characterized permeability enclosing the developing embryo. We report the automated dechorionation of 1600 embryos at once at 4 h postfertilization (hpf) and placement of the dechorionated embryos into 96-well plates for exposure by 6 hpf. The process removed ≥95% of the embryos from their chorions with 2% embryo mortality by 24 hpf, and 2% of the embryos malformed at 120 hpf. The robotic embryo placement allocated 6-hpf embryos to 94.7% ± 4.2% of the wells in multiple 96-well trials. The rate of embryo mortality was 2.8% (43 of 1536) from robotic handling, the rate of missed wells was 1.2% (18 of 1536), and the frequency of multipicks was <0.1%. Embryo malformations observed at 24 hpf occurred nearly twice as frequently from robotic handling (16 of 864; 1.9%) as from manual pipetting (9 of 864; 1%). There was no statistical difference between the success of performing the embryo placement robotically or manually.
- Subjects :
- Animals
Drug Evaluation, Preclinical instrumentation
Drug Evaluation, Preclinical methods
Embryonic Development drug effects
Robotics trends
Single Embryo Transfer methods
Zebrafish
Automation, Laboratory
Chorion metabolism
Drug Discovery
High-Throughput Screening Assays
Single Embryo Transfer instrumentation
Toxicity Tests
Subjects
Details
- Language :
- English
- ISSN :
- 2211-0690
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of laboratory automation
- Publication Type :
- Academic Journal
- Accession number :
- 22357610
- Full Text :
- https://doi.org/10.1177/2211068211432197