Back to Search
Start Over
Molecular characterization of extended-spectrum-β-lactamase-producing and plasmid-mediated AmpC β-lactamase-producing Escherichia coli isolated from stray dogs in South Korea.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2012 May; Vol. 56 (5), pp. 2705-12. Date of Electronic Publication: 2012 Feb 21. - Publication Year :
- 2012
-
Abstract
- A total of 47 extended-spectrum-cephalosporin-resistant Escherichia coli strains isolated from stray dogs in 2006 and 2007 in the Republic of Korea were investigated using molecular methods. Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase phenotypes were identified in 12 and 23 E. coli isolates, respectively. All 12 ESBL-producing isolates carried bla(CTX-M) genes. The most common CTX-M types were CTX-M-14 (n = 5) and CTX-M-24 (n = 3). Isolates producing CTX-M-3, CTX-M-55, CTX-M-27, and CTX-M-65 were also identified. Twenty-one of 23 AmpC β-lactamase-producing isolates were found to carry bla(CMY-2) genes. TEM-1 was associated with CTX-M and CMY-2 β-lactamases in 4 and 15 isolates, respectively. In addition to bla(TEM-1), two isolates carried bla(DHA-1), and one of them cocarried bla(CMY-2). Both CTX-M and CMY-2 genes were located on large (40 to 170 kb) conjugative plasmids that contained the insertion sequence ISEcp1 upstream of the bla genes. Only in the case of CTX-M genes was there an IS903 sequence downstream of the gene. The spread of ESBLs and AmpC β-lactamases occurred via both horizontal gene transfer, accounting for much of the CTX-M gene dissemination, and clonal spread, accounting for CMY-2 gene dissemination. The horizontal dissemination of bla(CTX-M) and bla(CMY-2) genes was mediated by IncF and IncI1-Iγ plasmids, respectively. The clonal spread of bla(CMY-2) was driven mainly by E. coli strains of virulent phylogroup D lineage ST648. To our knowledge, this is the first report of bla(DHA-1) in E. coli strains isolated from companion animals. This study also represents the first report of CMY-2 β-lactamase-producing E. coli isolates from dogs in the Republic of Korea.
- Subjects :
- Animals
Animals, Wild
Cephalosporin Resistance drug effects
Dogs
Electrophoresis, Gel, Pulsed-Field
Escherichia coli classification
Escherichia coli drug effects
Escherichia coli isolation & purification
Escherichia coli Infections drug therapy
Escherichia coli Infections microbiology
Gene Transfer, Horizontal
Microbial Sensitivity Tests
Phylogeny
Republic of Korea
Anti-Bacterial Agents pharmacology
Cephalosporin Resistance genetics
Cephalosporins pharmacology
Dog Diseases microbiology
Escherichia coli genetics
Escherichia coli Infections veterinary
Plasmids genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 56
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 22354297
- Full Text :
- https://doi.org/10.1128/AAC.05598-11