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A synthetic Escherichia coli system identifies a conserved origin tethering factor in Actinobacteria.
A synthetic Escherichia coli system identifies a conserved origin tethering factor in Actinobacteria.
- Source :
-
Molecular microbiology [Mol Microbiol] 2012 Apr; Vol. 84 (1), pp. 105-16. Date of Electronic Publication: 2012 Feb 28. - Publication Year :
- 2012
-
Abstract
- In eukaryotic and prokaryotic cells the establishment and maintenance of cell polarity is essential for numerous biological processes. In some bacterial species, the chromosome origins have been identified as molecular markers of cell polarity and polar chromosome anchoring factors have been identified, for example in Caulobacter crescentus. Although speculated, polar chromosome tethering factors have not been identified for Actinobacteria, to date. Here, using a minimal synthetic Escherichia coli system, biochemical and in vivo experiments, we provide evidence that Corynebacterium glutamicum cells tether the chromosome origins at the cell poles through direct physical interactions between the ParB-parS chromosomal centromere and the apical growth determinant DivIVA. The interaction between ParB and DivIVA proteins was also shown for other members of the Actinobacteria phylum, including Mycobacterium tuberculosis and Streptomyces coelicolor.<br /> (© 2012 Blackwell Publishing Ltd.)
- Subjects :
- Actinobacteria genetics
Bacterial Proteins genetics
Cell Cycle Proteins genetics
DNA, Bacterial genetics
Escherichia coli genetics
Point Mutation
Bacterial Proteins metabolism
Cell Cycle Proteins metabolism
Cell Polarity
Centromere genetics
Chromosomes, Bacterial genetics
Corynebacterium glutamicum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2958
- Volume :
- 84
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 22340668
- Full Text :
- https://doi.org/10.1111/j.1365-2958.2012.08011.x