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A synthetic Escherichia coli system identifies a conserved origin tethering factor in Actinobacteria.

A synthetic Escherichia coli system identifies a conserved origin tethering factor in Actinobacteria.

Authors :
Donovan C
Sieger B
Krämer R
Bramkamp M
Source :
Molecular microbiology [Mol Microbiol] 2012 Apr; Vol. 84 (1), pp. 105-16. Date of Electronic Publication: 2012 Feb 28.
Publication Year :
2012

Abstract

In eukaryotic and prokaryotic cells the establishment and maintenance of cell polarity is essential for numerous biological processes. In some bacterial species, the chromosome origins have been identified as molecular markers of cell polarity and polar chromosome anchoring factors have been identified, for example in Caulobacter crescentus. Although speculated, polar chromosome tethering factors have not been identified for Actinobacteria, to date. Here, using a minimal synthetic Escherichia coli system, biochemical and in vivo experiments, we provide evidence that Corynebacterium glutamicum cells tether the chromosome origins at the cell poles through direct physical interactions between the ParB-parS chromosomal centromere and the apical growth determinant DivIVA. The interaction between ParB and DivIVA proteins was also shown for other members of the Actinobacteria phylum, including Mycobacterium tuberculosis and Streptomyces coelicolor.<br /> (© 2012 Blackwell Publishing Ltd.)

Details

Language :
English
ISSN :
1365-2958
Volume :
84
Issue :
1
Database :
MEDLINE
Journal :
Molecular microbiology
Publication Type :
Academic Journal
Accession number :
22340668
Full Text :
https://doi.org/10.1111/j.1365-2958.2012.08011.x