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Assaying macrophage activity in a murine model of inflammatory bowel disease using fluorine-19 MRI.
- Source :
-
Laboratory investigation; a journal of technical methods and pathology [Lab Invest] 2012 Apr; Vol. 92 (4), pp. 636-45. Date of Electronic Publication: 2012 Feb 13. - Publication Year :
- 2012
-
Abstract
- Macrophages have an important role in the pathogenesis of most chronic inflammatory diseases. A means of non-invasively quantifying macrophage migration would contribute significantly towards our understanding of chronic inflammatory processes and aid the evaluation of novel therapeutic strategies. We describe the use of a perfluorocarbon tracer reagent and in vivo (19)F magnetic resonance imaging (MRI) to quantify macrophage burden longitudinally. We apply these methods to evaluate the severity and three-dimensional distribution of macrophages in a murine model of inflammatory bowel disease (IBD). MRI results were validated by histological analysis, immunofluorescence and quantitative real-time polymerase chain reaction. Selective depletion of macrophages in vivo was also performed, further validating that macrophage accumulation of perfluorocarbon tracers was the basis of (19)F MRI signals observed in the bowel. We tested the effects of two common clinical drugs, dexamethasone and cyclosporine A, on IBD progression. Whereas cyclosporine A provided mild therapeutic effect, unexpectedly dexamethasone enhanced colon inflammation, especially in the descending colon. Overall, (19)F MRI can be used to evaluate early-stage inflammation in IBD and is suitable for evaluating putative therapeutics. Due to its high macrophage specificity and quantitative ability, we envisage (19)F MRI having an important role in evaluating a wide range of chronic inflammatory conditions mediated by macrophages.
- Subjects :
- Animals
Anti-Inflammatory Agents therapeutic use
Colon immunology
Colon pathology
Cyclosporine therapeutic use
Dexamethasone therapeutic use
Female
Hyperplasia
Immunosuppressive Agents therapeutic use
Inflammatory Bowel Diseases diagnosis
Inflammatory Bowel Diseases drug therapy
Interleukin-10 genetics
Intestinal Mucosa pathology
Magnetic Resonance Imaging
Mice
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Confocal
Real-Time Polymerase Chain Reaction
Fluorine
Fluorocarbons
Inflammatory Bowel Diseases immunology
Macrophages physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1530-0307
- Volume :
- 92
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Laboratory investigation; a journal of technical methods and pathology
- Publication Type :
- Academic Journal
- Accession number :
- 22330343
- Full Text :
- https://doi.org/10.1038/labinvest.2012.7