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Low macrophage accumulation in skeletal muscle of obese type 2 diabetics and elderly subjects.
- Source :
-
Obesity (Silver Spring, Md.) [Obesity (Silver Spring)] 2012 Jul; Vol. 20 (7), pp. 1530-3. Date of Electronic Publication: 2012 Feb 08. - Publication Year :
- 2012
-
Abstract
- In addition to adipose tissue, recent studies suggest that skeletal muscle may also be a source of low-grade inflammation, particularly in inactive and/or overweight individuals. The aim of this study was to examine the presence of macrophages in skeletal muscle from obese subjects with type 2 diabetes (T2D) before and after a 9-month exercise program (vs. a non-exercising control group) (Study 1) and in young vs. elderly subjects (Study 2). In both studies, CD68(+) macrophages in vastus lateralis biopsies were determined by immunohistochemistry and inflammation gene expression measured. Macrophage content (%) was calculated by the number of macrophages per 100 muscle fibers. In Study 1, we found relatively low numbers (2-3%) of CD68(+) macrophages in skeletal muscle in obese T2D subjects (BMI = 37.3 ± 5.2 kg/m(2)), which were unchanged after a 9-month exercise program (P = 0.42). Similarly, in Study 2 (BMI = 27.1 ± 2.5 kg/m(2)), CD68(+) macrophages were relatively low in muscle (4-5%) and were not different between young and elderly individuals (P = 0.42). However, elderly subjects had twofold higher CD68 and CD206 gene expression (both P < 0.002) than young participants. In both studies, CD68(+) muscle macrophages were not associated with BMI. In conclusion, we found little evidence of macrophage accumulation in skeletal muscle in obese T2D subjects or in elderly individuals. A 9-month exercise program was not associated with a decrease in macrophage content.
- Subjects :
- Adult
Aged
Aged, 80 and over
Aging genetics
Cross-Sectional Studies
Diabetes Mellitus, Type 2 genetics
Exercise Therapy methods
Female
Gene Expression
Glucose Tolerance Test
Humans
Immunohistochemistry
Inflammation genetics
Inflammation metabolism
Inflammation pathology
Male
Middle Aged
Obesity genetics
Young Adult
Aging metabolism
Antigens, CD metabolism
Antigens, Differentiation, Myelomonocytic metabolism
Blood Glucose metabolism
Diabetes Mellitus, Type 2 metabolism
Macrophages metabolism
Muscle, Skeletal pathology
Obesity pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1930-739X
- Volume :
- 20
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Obesity (Silver Spring, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 22314623
- Full Text :
- https://doi.org/10.1038/oby.2012.24