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Platelet-rich plasma inhibits the apoptosis of highly adipogenic homogeneous preadipocytes in an in vitro culture system.
- Source :
-
Experimental & molecular medicine [Exp Mol Med] 2012 May 31; Vol. 44 (5), pp. 330-9. - Publication Year :
- 2012
-
Abstract
- Auto-transplantation of adipose tissue is commonly used for the treatment of tissue defects in plastic surgery. The survival of the transplanted adipose tissue is not always constant, and one of reasons is the accelerated apoptosis of the implanted preadipocytes. We have recently established highly homogeneous preadipocytes, named ccdPAs. The aim of the current study was to evaluate the regulation of the potency of platelet-rich plasma (PRP) on the apoptosis of ccdPAs in vitro. PRP stimulated the proliferation of the preadipocytes in a dose-dependent manner, and the stimulatory activity of 2% PRP was significantly higher than that of 2% FBS or 2% platelet-poor plasma (PPP). The presence of 2% PRP significantly inhibited serum starvation- or TNF-α/cycloheximide-induced apoptosis in comparison to 2% FBS or 2% PPP. DAPK1 and Bcl-2-interacting mediator of cell death (BIM) mRNAs were reduced in the preadipocytes cultured with 2% PRP in comparison to those cultured in 2% FBS. The gene expression levels were significantly higher in cells cultured without serum in comparison to cells cultured with 2% FBS, and the levels in the cells with 2% PRP were reduced to 5-10% of those in the cells without serum. These results indicated that ccdPAs exhibit anti- apoptotic activities, in addition to increased proliferation, when cultured in 2% PRP in comparison to the same concentration of FBS, and that this was accompanied with reduced levels of DAPK1 and BIM mRNA expression in in vitro culture. PRP may improve the outcome of transplantation of adipose tissue by enhancing the anti-apoptotic activities of the implanted preadipocytes.
- Subjects :
- Adipose Tissue cytology
Adipose Tissue metabolism
Apoptosis Regulatory Proteins antagonists & inhibitors
Apoptosis Regulatory Proteins metabolism
Bcl-2-Like Protein 11
Calcium-Calmodulin-Dependent Protein Kinases antagonists & inhibitors
Calcium-Calmodulin-Dependent Protein Kinases metabolism
Cell Proliferation
Cells, Cultured
Death-Associated Protein Kinases
Gene Expression Regulation
Humans
Membrane Proteins antagonists & inhibitors
Membrane Proteins metabolism
Proto-Oncogene Proteins antagonists & inhibitors
Proto-Oncogene Proteins metabolism
Tissue Transplantation
Adipocytes cytology
Apoptosis physiology
Cell Culture Techniques methods
Cell Differentiation
Platelet-Rich Plasma metabolism
Platelet-Rich Plasma physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2092-6413
- Volume :
- 44
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Experimental & molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 22314197
- Full Text :
- https://doi.org/10.3858/emm.2012.44.5.037