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Evaluation of direct in vivo gene transfer in an equine metacarpal IV ostectomy model using an adenoviral vector encoding the bone morphogenetic protein-2 and protein-7 gene.
- Source :
-
Veterinary surgery : VS [Vet Surg] 2012 Apr; Vol. 41 (3), pp. 345-54. Date of Electronic Publication: 2012 Feb 06. - Publication Year :
- 2012
-
Abstract
- Objective: To evaluate gene transfer in an equine metacarpal IV (MCIV) ostectomy model using adenoviral vectors encoding the human bone morphogenetic protein-2 and protein-7 gene (Ad-BMP-2/-7).<br />Experimental Animals: Healthy adult horses (n = 15).<br />Methods: A plate stabilized, critical size 1.5 cm ostectomy was created in left and right MCIV. The ostectomy site was injected with either Ad-green fluorescent protein (Ad-GFP) or Ad-hBMP-2/-7 at completion of surgery; the same treatment was assigned to both the left and right forelimb of each horse (n = 5 horses/group). Bone healing was evaluated radiographically every 2 weeks for 16 weeks. Horses in a pilot study (n = 5) were used as untreated controls for radiographic evaluation to 8 weeks. After euthanasia at 16 weeks bone healing was evaluated using dual energy X-ray absorptiometry (DEXA) and histomorphometry. Data were analyzed using an ANOVA or Kruskal-Wallis test. Level of significance was P < .05.<br />Results: At 4 and 6 weeks, the Ad-GFP group had a significantly lower percentage defect ossification compared with the untreated control group. There was no significant difference between untreated and Ad-hBMP-2/-7 groups at any time point and no significant difference in bone healing radiographically, histologically, or using DEXA between any groups at 16 weeks.<br />Conclusions: Ad-hBMP-2/-7 did not improve bone healing in horses at 16 weeks.<br /> (© Copyright 2012 by The American College of Veterinary Surgeons.)
- Subjects :
- Absorptiometry, Photon veterinary
Animals
Genetic Vectors genetics
Horses surgery
Male
Metacarpal Bones diagnostic imaging
Metacarpal Bones pathology
Metacarpal Bones surgery
Bone Morphogenetic Protein 2 genetics
Bone Morphogenetic Protein 7 genetics
Bone Regeneration physiology
Gene Transfer Techniques veterinary
Metacarpal Bones physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-950X
- Volume :
- 41
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Veterinary surgery : VS
- Publication Type :
- Academic Journal
- Accession number :
- 22308976
- Full Text :
- https://doi.org/10.1111/j.1532-950X.2011.00947.x