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Functional melanocortin-2 receptors are expressed by mouse aorta-derived mesenchymal progenitor cells.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 2012 May 15; Vol. 355 (1), pp. 60-70. Date of Electronic Publication: 2012 Jan 27. - Publication Year :
- 2012
-
Abstract
- A local melanocortin system is active during tissue injury and inflammation. Thus far this system has been described as autocrine in nature where local production of pro-opiomelanocortin (POMC) peptides by leukocytes feeds back on melanocortin receptor (MC-R) expressing immune cells to quell inflammatory cytokine production. Here we present evidence that POMC peptides may generate extracellular matrix (ECM) changes by inducing matrix production by cells of the mesenchymal lineage through activation of the MC2-R. Using immunoblot, we determined that mouse aorta-derived mesenchymal progenitor cells express both MC2-R and MC3-R. These progenitors respond to treatment with ACTH by increasing collagen matrix synthesis as assessed by picrosirius red stain and (3)H-proline incorporation. ACTH also induces transient increases in intracellular calcium ([Ca(2+)](i)) as assessed using the fluorescent Ca(2+) indicator, fura-2. The ACTH-induced changes in [Ca(2+)](i) are consistent with MC2-R signaling and consist of both an intracellular release and an extracellular influx of Ca(2+). Both mouse aortic mesenchymal progenitors and mouse macrophage cells express POMC and the prohormone convertase 1/3 (PC1/3) indicating they have the potential to contribute to the local production of POMC peptides. These data demonstrate functional MC2-R expression in mouse aorta-derived mesenchymal progenitors and implicate both macrophage and mesenchymal cells as relevant sources of local POMC peptides.<br /> (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Adrenocorticotropic Hormone pharmacology
Animals
Aorta cytology
Aorta drug effects
Azo Compounds
Calcium metabolism
Cells, Cultured
Collagen genetics
Collagen metabolism
Extracellular Matrix drug effects
Fura-2
Gene Expression drug effects
Macrophages cytology
Macrophages drug effects
Macrophages metabolism
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells drug effects
Mice
Mice, Inbred C57BL
Pro-Opiomelanocortin genetics
Pro-Opiomelanocortin metabolism
Proprotein Convertase 1 genetics
Proprotein Convertase 1 metabolism
Rats
Rats, Inbred WKY
Receptor, Melanocortin, Type 2 genetics
Receptor, Melanocortin, Type 3 genetics
Signal Transduction drug effects
Aorta metabolism
Mesenchymal Stem Cells metabolism
Receptor, Melanocortin, Type 2 metabolism
Receptor, Melanocortin, Type 3 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8057
- Volume :
- 355
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 22306084
- Full Text :
- https://doi.org/10.1016/j.mce.2012.01.019