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In vivo protein crystallization opens new routes in structural biology.
- Source :
-
Nature methods [Nat Methods] 2012 Jan 29; Vol. 9 (3), pp. 259-62. Date of Electronic Publication: 2012 Jan 29. - Publication Year :
- 2012
-
Abstract
- Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
Details
- Language :
- English
- ISSN :
- 1548-7105
- Volume :
- 9
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nature methods
- Publication Type :
- Academic Journal
- Accession number :
- 22286384
- Full Text :
- https://doi.org/10.1038/nmeth.1859