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Measurement of genome-wide RNA synthesis and decay rates with Dynamic Transcriptome Analysis (DTA).
- Source :
-
Bioinformatics (Oxford, England) [Bioinformatics] 2012 Mar 15; Vol. 28 (6), pp. 884-5. Date of Electronic Publication: 2012 Jan 28. - Publication Year :
- 2012
-
Abstract
- Standard transcriptomics measures total cellular RNA levels. Our understanding of gene regulation would be greatly improved if we could measure RNA synthesis and decay rates on a genome-wide level. To that end, the Dynamic Transcriptome Analysis (DTA) method has been developed. DTA combines metabolic RNA labeling with standard transcriptomics to measure RNA synthesis and decay rates in a precise and non-perturbing manner. Here, we present the open source R/Bioconductor software package DTA. It implements all required bioinformatics steps that allow the accurate absolute quantification and comparison of RNA turnover.
Details
- Language :
- English
- ISSN :
- 1367-4811
- Volume :
- 28
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Bioinformatics (Oxford, England)
- Publication Type :
- Academic Journal
- Accession number :
- 22285829
- Full Text :
- https://doi.org/10.1093/bioinformatics/bts052