A cis-complex of NB-2/contactin-5 with amyloid precursor-like protein 1 is localized on the presynaptic membrane.

Authors :: Shimoda Y
Koseki F
Itoh M
Toyoshima M
Watanabe K
Source :: Neuroscience letters [Neurosci Lett] 2012 Feb 29; Vol. 510 (2), pp. 148-53. Date of Electronic Publication: 2012 Jan 21.
Publication Year :: 2012
Abstract: NB-2/contactin-5 plays an important role in synapse formation in the developing auditory system of rodents. In this study, to further elucidate the molecular role of NB-2 in synapse formation, we examined the interaction between NB-2 and amyloid precursor-like protein 1 (APLP1), as well as their possible co-localization at the synapse. Pull-down assays and cell surface binding assays demonstrated that NB-2 interacts with APLP1. Furthermore, the protein expression profile of APLP1 in western blots was similar to that of NB-2, and localization of APLP1 mRNA partially overlapped that of NB-2 mRNA. In cultured hippocampal neurons, immunofluorescence signals for both NB-2 and APLP1 overlapped with synapsin, a presynaptic marker. Biochemical analysis showed that both NB-2 and APLP1 were enriched in the presynaptic fraction. These results indicate that NB-2 forms a cis-complex with APLP1 on the presynaptic membrane.<br /> (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)

Subjects :: Animals
Blotting, Western
Brain growth & development
Cells, Cultured
Fluorescent Antibody Technique
Hippocampus metabolism
In Situ Hybridization
Mice
Mice, Inbred C57BL
Neurons metabolism
Presynaptic Terminals metabolism
Recombinant Fusion Proteins metabolism
Amyloid beta-Protein Precursor metabolism
Brain metabolism
Cell Adhesion Molecules, Neuronal metabolism
Synaptic Membranes metabolism

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