Suppression of nitric oxide production on LPS/IFN-γ-stimulated RAW264.7 macrophages by a novel catechin, pilosanol N, from Agrimonia pilosa Ledeb.

Authors :: Taira J
Ohmine W
Ogi T
Nanbu H
Ueda K
Source :: Bioorganic & medicinal chemistry letters [Bioorg Med Chem Lett] 2012 Feb 15; Vol. 22 (4), pp. 1766-9. Date of Electronic Publication: 2012 Jan 03.
Publication Year :: 2012
Abstract: A novel catechin, pilosanol N (1), was isolated from Agrimonia pilosa Ledeb and its structure was determined by (1)H, (13)C NMR and HRESI-MS analyses. Compound 1 inhibited nitric oxide (NO) production in LPS/IFN-γ -induced RAW264.7 macrophages, and then the iNOS gene expression and its protein production with LPS/IFN-γ treatment cells were also suppressed in the presence of 1. In addition, compound 1 scavenged NO or nitrogen radicals generated from NOR3 (4-ethyl-2-hydroxyamino-5-nitro-3-hexenamide) as an NO donor. These results indicated that pilosanol N can decrease the level of NO through a mechanism that involved both a decrease in the NO production and NO scavenging.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Subjects :: Catechin chemistry
Catechin isolation & purification
Interferon-gamma chemistry
Interferon-gamma pharmacology
Lipopolysaccharides chemistry
Lipopolysaccharides pharmacology
Magnetic Resonance Spectroscopy
Molecular Structure
Nitric Oxide antagonists & inhibitors
Agrimonia chemistry
Catechin analogs & derivatives
Catechin pharmacology
Macrophages drug effects
Nitric Oxide biosynthesis

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