Back to Search
Start Over
Proteomic profiling of follicular and papillary thyroid tumors.
- Source :
-
European journal of endocrinology [Eur J Endocrinol] 2012 Apr; Vol. 166 (4), pp. 657-67. Date of Electronic Publication: 2012 Jan 24. - Publication Year :
- 2012
-
Abstract
- Objective: Thyroid proteomics is a new direction in thyroid cancer research aiming at etiological understanding and biomarker identification for improved diagnosis.<br />Methods: Two-dimensional electrophoresis was applied to cytosolic protein extracts from frozen thyroid samples (ten follicular adenomas, nine follicular carcinomas, ten papillary carcinomas, and ten reference thyroids). Spots with differential expression were revealed by image and multivariate statistical analyses, and identified by mass spectrometry.<br />Results: A set of 25 protein spots significant for discriminating between the sample groups was identified. Proteins identified for nine of these spots were studied further including 14-3-3 protein beta/alpha, epsilon, and zeta/delta, peroxiredoxin 6, selenium-binding protein 1, protein disulfide-isomerase precursor, annexin A5 (ANXA5), tubulin alpha-1B chain, and α1-antitrypsin precursor. This subset of protein spots carried the same predictive power in differentiating between follicular carcinoma and adenoma or between follicular and papillary carcinoma, as compared with the larger set of 25 spots. Protein expression in the sample groups was demonstrated by western blot analyses. For ANXA5 and the 14-3-3 proteins, expression in tumor cell cytoplasm was demonstrated by immunohistochemistry both in the sample groups and an independent series of papillary thyroid carcinomas.<br />Conclusion: The proteins identified confirm previous findings in thyroid proteomics, and suggest additional proteins as dysregulated in thyroid tumors.
- Subjects :
- Adenocarcinoma, Follicular
Biomarkers, Tumor analysis
Biomarkers, Tumor metabolism
Carcinoma
Carcinoma, Papillary
Diagnosis, Differential
Electrophoresis, Gel, Two-Dimensional methods
Humans
Immunohistochemistry
Mass Spectrometry
Predictive Value of Tests
Protein Array Analysis methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Thyroid Cancer, Papillary
Thyroid Neoplasms diagnosis
Thyroid Neoplasms pathology
Validation Studies as Topic
Proteomics methods
Thyroid Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1479-683X
- Volume :
- 166
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 22275472
- Full Text :
- https://doi.org/10.1530/EJE-11-0856