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Effect of Streptococcus salivarius K12 on the in vitro growth of Candida albicans and its protective effect in an oral candidiasis model.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2012 Apr; Vol. 78 (7), pp. 2190-9. Date of Electronic Publication: 2012 Jan 20. - Publication Year :
- 2012
-
Abstract
- Oral candidiasis is often accompanied by severe inflammation, resulting in a decline in the quality of life of immunosuppressed individuals and elderly people. To develop a new oral therapeutic option for candidiasis, a nonpathogenic commensal oral probiotic microorganism, Streptococcus salivarius K12, was evaluated for its ability to modulate Candida albicans growth in vitro, and its therapeutic activity in an experimental oral candidiasis model was tested. In vitro inhibition of mycelial growth of C. albicans was determined by plate assay and fluorescence microscopy. Addition of S. salivarius K12 to modified RPMI 1640 culture medium inhibited the adherence of C. albicans to the plastic petri dish in a dose-dependent manner. Preculture of S. salivarius K12 potentiated its inhibitory activity for adherence of C. albicans. Interestingly, S. salivarius K12 was not directly fungicidal but appeared to inhibit Candida adhesion to the substratum by preferentially binding to hyphae rather than yeast. To determine the potentially anti-infective attributes of S. salivarius K12 in oral candidiasis, the probiotic was administered to mice with orally induced candidiasis. Oral treatment with S. salivarius K12 significantly protected the mice from severe candidiasis. These findings suggest that S. salivarius K12 may inhibit the process of invasion of C. albicans into mucous surfaces or its adhesion to denture acrylic resins by mechanisms not associated with the antimicrobial activity of the bacteriocin. S. salivarius K12 may be useful as a probiotic as a protective tool for oral care, especially with regard to candidiasis.
- Subjects :
- Animals
Bacteriocins pharmacology
Candida albicans drug effects
Candida albicans isolation & purification
Candidiasis, Oral microbiology
Candidiasis, Oral pathology
Cell Adhesion drug effects
Disease Models, Animal
Female
Humans
Hyphae drug effects
Hyphae growth & development
Mice
Mice, Inbred ICR
Microbial Sensitivity Tests methods
Probiotics administration & dosage
Streptococcus classification
Streptococcus metabolism
Tongue microbiology
Treatment Outcome
Candida albicans growth & development
Candidiasis, Oral therapy
Probiotics pharmacology
Probiotics therapeutic use
Streptococcus growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5336
- Volume :
- 78
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 22267663
- Full Text :
- https://doi.org/10.1128/AEM.07055-11