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ImageStream promyelocytic leukemia protein immunolocalization: in search of promyelocytic leukemia cells.
- Source :
-
Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2012 Mar; Vol. 81 (3), pp. 232-7. Date of Electronic Publication: 2012 Jan 20. - Publication Year :
- 2012
-
Abstract
- Acute promyelocytic leukemia (APL) is a hematological emergency in which a rapid diagnosis is essential for early administration of appropriate therapy, including all-trans retinoic acid before the onset of fatal coagulopathy. Currently, the following methodologies are widely used for rapid initial diagnosis of APL: 1) identification of hypergranular leukemic promyelocytes by using classical morphology; 2) identification of cells with diffuse promyelocytic leukemia (PML) protein distribution by immunofluorescence microscopy; 3) evidence of aberrant promyelocyte surface immunophenotype by conventional flow cytometry (FCM). Here, we show a method for immunofluorescent detection of PML localization using ImageStream FCM. This technique provides objective per-cell quantitative image analysis for statistically large sample sizes, enabling precise and operator-independent PML pattern recognition even in electronic and real dilution experiments up to 10% of APL cellular presence. Therefore, we evidence that this method could be helpful for rapid and objective initial diagnosis and the prompt initiation of APL treatment.<br /> (Copyright © 2012 International Society for Advancement of Cytometry.)
- Subjects :
- Cell Line, Tumor
Humans
Neoplasm Proteins analysis
Neoplasm Proteins chemistry
Nuclear Proteins chemistry
Promyelocytic Leukemia Protein
Staining and Labeling methods
Tissue Fixation methods
Transcription Factors chemistry
Tumor Suppressor Proteins chemistry
Flow Cytometry methods
Granulocyte Precursor Cells physiology
Image Cytometry methods
Leukemia, Promyelocytic, Acute diagnosis
Nuclear Proteins analysis
Transcription Factors analysis
Tumor Suppressor Proteins analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1552-4930
- Volume :
- 81
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cytometry. Part A : the journal of the International Society for Analytical Cytology
- Publication Type :
- Academic Journal
- Accession number :
- 22266986
- Full Text :
- https://doi.org/10.1002/cyto.a.22013