Back to Search
Start Over
Monolithically integrated Mach-Zehnder biosensors for real-time label-free monitoring of biomolecular reactions.
- Source :
-
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference [Annu Int Conf IEEE Eng Med Biol Soc] 2011; Vol. 2011, pp. 7654-7. - Publication Year :
- 2011
-
Abstract
- Arrays of monolithically integrated Mach-Zehnder interferometers were fabricated by standard silicon technology and applied to the label-free real-time monitoring of biomolecular interactions. Chips accommodating 10 MZIs were functionalized with recognition biomolecules and encapsulated in wafer scale. Detection is based on Frequency-Resolved Mach-Zehnder Interferometry, a new concept that takes advantage of the broad-band input spectrum by monitoring the changes for every input frequency. The sensitivity of the device in terms of refractive index changes (Δn) was calculated using isopropanol/water solutions. A detection limit of Δn = 4 × 10(-6) was calculated. The bioanalytical capabilities of the device there demonstrated through model binding assays (biotin/streptavidin) as well as the detection of total prostate specific antigen in serum samples using devices coated with antigen-specific monoclonal antibody. Detection limits at the pM range were determined.
Details
- Language :
- English
- ISSN :
- 2694-0604
- Volume :
- 2011
- Database :
- MEDLINE
- Journal :
- Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
- Publication Type :
- Academic Journal
- Accession number :
- 22256111
- Full Text :
- https://doi.org/10.1109/IEMBS.2011.6091886