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Serum antibodies to whole-cell and recombinant antigens of Borrelia burgdorferi in cottontail rabbits.
- Source :
-
Journal of wildlife diseases [J Wildl Dis] 2012 Jan; Vol. 48 (1), pp. 12-20. - Publication Year :
- 2012
-
Abstract
- Archived serum samples, from 95 eastern cottontail rabbits (Sylvilagus floridanus) captured in New York, New York, USA and Millbrook, New York, USA, during 1985-86, were analyzed in solid-phase enzyme-linked immunosorbent assays (ELISA) for total and class-specific immunoglobulin (Ig) M antibodies to whole-cell or recombinant antigens of Borrelia burgdorferi sensu stricto. Using a polyvalent conjugate, rabbit sera contained antibodies to whole-cell and recombinant antigens (protein [p]35, p37, or VlsE) during different seasons, but there was no reactivity to outer surface protein (Osp)A or OspB. Seventy-six of the 102 sera (75%) analyzed were reactive with one or more of the antigens; 61 of the positive samples (80%) reacted to whole-cell antigens, followed by results for the p35 (58%, 44/76), VlsE (43%, 33/76), and p37 (29%, 22/ 76) antigens. Fifty-eight sera (76%) contained antibodies to the VlsE or p35 antigens with or without reactivity to whole-cell antigens. High antibody titers (≥1:2,560) recorded for 52 sera indicate robust antibody production. In analyses for IgM antibodies in an ELISA containing whole-cell antigens, there were 30 positive sera; titers ranged from 1:160 to 1:640. There was minimal cross-reactivity when rabbit antisera to Treponema pallidum or four serovars of Leptospira interrogans were screened against B. burgdorferi antigens. Based on more-specific results, VlsE and p35 antigens appear to be useful markers for detecting possible B. burgdorferi infections.
- Subjects :
- Animals
Antibodies, Bacterial immunology
Cross Reactions
Enzyme-Linked Immunosorbent Assay veterinary
Female
Lyme Disease diagnosis
Lyme Disease epidemiology
Lyme Disease immunology
Lyme Disease veterinary
Male
New York epidemiology
Rabbits immunology
Recombinant Proteins immunology
Antibodies, Bacterial blood
Antigens, Bacterial immunology
Borrelia burgdorferi immunology
Rabbits microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1943-3700
- Volume :
- 48
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of wildlife diseases
- Publication Type :
- Academic Journal
- Accession number :
- 22247369
- Full Text :
- https://doi.org/10.7589/0090-3558-48.1.12