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MICS-1 interacts with mitochondrial ATAD-3 and modulates lifespan in C. elegans.
MICS-1 interacts with mitochondrial ATAD-3 and modulates lifespan in C. elegans.
- Source :
-
Experimental gerontology [Exp Gerontol] 2012 Mar; Vol. 47 (3), pp. 270-5. Date of Electronic Publication: 2012 Jan 05. - Publication Year :
- 2012
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Abstract
- Caenorhabditis elegans open reading frame T21C9.1 encodes an uncharacterized protein, which is here named MICS-1 (mitochondrial scaffolding protein-1). It is predicted to be the homolog of human outer mitochondrial membrane protein 25 (OMP25 or synaptojanin-2-binding protein), which is a PDZ domain containing protein with a putative role in cellular stress response pathways. Here, we provide evidence that MICS-1 is an interacting partner of mitochondrial protein ATAD-3 (homologue of human ATAD3), which is essential for C. elegans development. We demonstrate that mics-1(RNAi) animals or mics-1 mutants display enhanced longevity with an increased mean lifespan of up to 54% compared to control animals. Of note, also atad-3(RNAi) promoted longevity, although to a lesser extend (29% compared to controls). In addition, thermal stress of mics-1 mutants induced low reactive oxygen species (ROS) production, whereas atad-3(RNAi) animals were highly sensitive to this assay, displaying drastically increased ROS levels. Further studies revealed that MICS-1 and ATAD-3 associated longevity was partially dependent on the presence of DAF-16. However, for both conditions, we also found a DAF-16 independent extension of lifespan. Finally, we observed an additional lifespan extension in mics-1 mutants when subjected to atad-3(RNAi) whereas heat induced ROS production was even aggravated under this condition. This suggests (partially) independent effects of MICS-1 and ATAD-3 on lifespan and ROS production in vivo.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Caenorhabditis elegans genetics
Caenorhabditis elegans Proteins genetics
Caenorhabditis elegans Proteins metabolism
Carrier Proteins genetics
Carrier Proteins metabolism
Hot Temperature
Mitochondrial Proteins genetics
Mitochondrial Proteins metabolism
Mutation
Protein Binding physiology
Reactive Oxygen Species metabolism
Caenorhabditis elegans metabolism
Caenorhabditis elegans Proteins physiology
Carrier Proteins physiology
Longevity physiology
Mitochondria metabolism
Mitochondrial Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6815
- Volume :
- 47
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Experimental gerontology
- Publication Type :
- Academic Journal
- Accession number :
- 22245785
- Full Text :
- https://doi.org/10.1016/j.exger.2011.12.011