Structural analysis of capsular polysaccharides expressed by Burkholderia mallei and Burkholderia pseudomallei.

Authors :: Heiss C
Burtnick MN
Wang Z
Azadi P
Brett PJ
Source :: Carbohydrate research [Carbohydr Res] 2012 Feb 15; Vol. 349, pp. 90-4. Date of Electronic Publication: 2011 Dec 23.
Publication Year :: 2012
Abstract: Capsular polysaccharides (CPSs) were isolated from O-polysaccharide deficient strains of Burkholderia mallei and Burkholderia pseudomallei using a modified hot phenol/water extraction procedure. Glycosyl composition, methylation, MALDI-TOF MS analyses as well as (1)H NMR spectroscopy including COSY, TOCSY, NOESY, HMBC and HSQC experiments identified the presence of two distinct CPS antigens in the samples exhibiting the following structures: This study confirms the ability of B. mallei to express a 6-deoxy-heptan CPS and represents the first report of a mannan CPS being expressed by these bacterial pathogens.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Subjects :: Bacterial Capsules chemistry
Burkholderia mallei growth & development
Burkholderia pseudomallei growth & development
Carbohydrate Conformation
Nuclear Magnetic Resonance, Biomolecular
Polysaccharides isolation & purification
Bacterial Capsules genetics
Burkholderia mallei genetics
Burkholderia pseudomallei genetics
Polysaccharides chemistry
Polysaccharides genetics

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