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Tolerogenic DNA vaccine for prevention of autoimmune ovarian disease.
- Source :
-
Immunological investigations [Immunol Invest] 2012; Vol. 41 (3), pp. 249-60. Date of Electronic Publication: 2012 Jan 05. - Publication Year :
- 2012
-
Abstract
- DNA vaccines have been widely used to induce immune responses against molecular targets. In this study, we explored the possibility of using DNA vaccine combined with the immunosuppressant FK506 (tacrolimus) to antigen-specifically suppress unwanted immune responses and prevent autoimmune ovarian disease. To that end, we immunized C57BL/6 mice with a DNA vaccine encoding mouse zona pellucida 3 (ZP3) together with FK506. The immunization induced ZP3-specific CD4(+)CD25(+)Foxp3(+) regulatory T cells (Treg), which suppressed the induction of ZP3-specific delayed-type hypersensitivity in the animals. Significantly, the immunization also protected the animals from experimentally induced autoimmune ovarian disease. These results suggest that DNA vaccination in the presence of FK506 may be used to induce Treg cells and prevent AOD.
- Subjects :
- Animals
Autoimmune Diseases immunology
CD4 Antigens metabolism
Cells, Cultured
Egg Proteins administration & dosage
Egg Proteins genetics
Egg Proteins immunology
Female
Forkhead Transcription Factors metabolism
Hypersensitivity, Delayed chemically induced
Hypersensitivity, Delayed prevention & control
Immune Tolerance
Immunization
Immunosuppressive Agents administration & dosage
Immunosuppressive Agents adverse effects
Interleukin-2 Receptor alpha Subunit metabolism
Membrane Glycoproteins administration & dosage
Membrane Glycoproteins genetics
Membrane Glycoproteins immunology
Mice
Mice, Inbred C57BL
Ovarian Diseases immunology
Receptors, Cell Surface administration & dosage
Receptors, Cell Surface genetics
Receptors, Cell Surface immunology
T-Lymphocytes, Regulatory drug effects
T-Lymphocytes, Regulatory immunology
T-Lymphocytes, Regulatory pathology
Tacrolimus administration & dosage
Tacrolimus adverse effects
Zona Pellucida Glycoproteins
Autoimmune Diseases prevention & control
Egg Proteins metabolism
Hypersensitivity, Delayed immunology
Membrane Glycoproteins metabolism
Ovarian Diseases prevention & control
Receptors, Cell Surface metabolism
T-Lymphocytes, Regulatory metabolism
Vaccines, DNA administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1532-4311
- Volume :
- 41
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Immunological investigations
- Publication Type :
- Academic Journal
- Accession number :
- 22221010
- Full Text :
- https://doi.org/10.3109/08820139.2011.622828