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Simplified synthetic antibody libraries.
- Source :
-
Methods in enzymology [Methods Enzymol] 2012; Vol. 502, pp. 3-23. - Publication Year :
- 2012
-
Abstract
- Synthetic antibody libraries are constructed from scratch using designed synthetic DNA. Precise control over design enables the use of highly optimized human frameworks and the introduction of defined chemical diversity at positions that are most likely to contribute to antigen recognition. We describe complete methods for the design, construction, and application of simplified synthetic antibody libraries built on a single human framework with diversity restricted to four complementarity-determining regions and two amino acids (tyrosine and serine). Despite the extreme simplicity of design, these libraries are capable of generating specific antibodies against diverse protein antigens. Moreover, the same methods can be used to build more complex libraries that can produce synthetic antibodies with affinities and specificities beyond the scope of natural antibodies. Most importantly, these simplified methods rely on standard supplies, equipment, and methods that are accessible to any molecular biology laboratory.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Amino Acids genetics
Antibodies genetics
Antibodies immunology
Antibodies metabolism
Antibody Affinity immunology
Antigens genetics
Antigens immunology
Bacteriophages chemistry
Biotinylation
Electroporation
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Humans
Models, Molecular
Molecular Sequence Data
Plasmids chemistry
Sequence Analysis, DNA
Transformation, Bacterial
Amino Acids immunology
Antibodies chemistry
Antigens metabolism
Bacteriophages genetics
Peptide Library
Plasmids genetics
Protein Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1557-7988
- Volume :
- 502
- Database :
- MEDLINE
- Journal :
- Methods in enzymology
- Publication Type :
- Academic Journal
- Accession number :
- 22208979
- Full Text :
- https://doi.org/10.1016/B978-0-12-416039-2.00001-X