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DNA-binding activity of adeno-associated virus Rep is required for inverted terminal repeat-dependent complex formation with herpes simplex virus ICP8.
- Source :
-
Journal of virology [J Virol] 2012 Mar; Vol. 86 (5), pp. 2859-63. Date of Electronic Publication: 2011 Dec 28. - Publication Year :
- 2012
-
Abstract
- Herpes simplex virus (HSV) helper functions for (AAV) replication comprise HSV ICP8 and helicase-primase UL5/UL52/UL8. Here we show that N-terminal amino acids of AAV Rep78 that contact the Rep-binding site within the AAV inverted terminal repeat (ITR) are required for ternary-complex formation with infected-cell protein 8 (ICP8) on AAV single-strand DNA (ssDNA) in vitro and for colocalization in nuclear replication domains in vivo. Our data suggest that HSV-dependent AAV replication is initiated by Rep contacting the AAV ITR and by cooperative binding of ICP8 on AAV ssDNA.
- Subjects :
- Binding Sites
DNA Helicases chemistry
DNA Helicases genetics
DNA Replication
DNA-Binding Proteins genetics
Dependovirus chemistry
Dependovirus genetics
Dependovirus physiology
HeLa Cells
Helper Viruses genetics
Helper Viruses metabolism
Humans
Parvoviridae Infections virology
Protein Structure, Tertiary
Protein Transport
Simplexvirus genetics
Viral Proteins chemistry
Viral Proteins genetics
Virus Replication
DNA Helicases metabolism
DNA-Binding Proteins metabolism
Dependovirus enzymology
Simplexvirus metabolism
Terminal Repeat Sequences
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 86
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 22205745
- Full Text :
- https://doi.org/10.1128/JVI.06364-11