Back to Search
Start Over
The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing.
- Source :
-
PloS one [PLoS One] 2011; Vol. 6 (12), pp. e29174. Date of Electronic Publication: 2011 Dec 16. - Publication Year :
- 2011
-
Abstract
- NIP7 is one of the many trans-acting factors required for eukaryotic ribosome biogenesis, which interacts with nascent pre-ribosomal particles and dissociates as they complete maturation and are exported to the cytoplasm. By using conditional knockdown, we have shown previously that yeast Nip7p is required primarily for 60S subunit synthesis while human NIP7 is involved in the biogenesis of 40S subunit. This raised the possibility that human NIP7 interacts with a different set of proteins as compared to the yeast protein. By using the yeast two-hybrid system we identified FTSJ3, a putative ortholog of yeast Spb1p, as a human NIP7-interacting protein. A functional association between NIP7 and FTSJ3 is further supported by colocalization and coimmunoprecipitation analyses. Conditional knockdown revealed that depletion of FTSJ3 affects cell proliferation and causes pre-rRNA processing defects. The major pre-rRNA processing defect involves accumulation of the 34S pre-rRNA encompassing from site A' to site 2b. Accumulation of this pre-rRNA indicates that processing of sites A0, 1 and 2 are slower in cells depleted of FTSJ3 and implicates FTSJ3 in the pathway leading to 18S rRNA maturation as observed previously for NIP7. The results presented in this work indicate a close functional interaction between NIP7 and FTSJ3 during pre-rRNA processing and show that FTSJ3 participates in ribosome synthesis in human cells.
- Subjects :
- Cell Nucleolus drug effects
Cell Nucleolus metabolism
Cell Proliferation drug effects
Doxycycline pharmacology
Gene Knockdown Techniques
HEK293 Cells
Humans
Immunoprecipitation
Kinetics
Methyltransferases genetics
Methyltransferases isolation & purification
Nuclear Proteins genetics
Nuclear Proteins isolation & purification
Protein Binding drug effects
Protein Transport drug effects
RNA metabolism
RNA Interference drug effects
RNA, Messenger genetics
RNA, Messenger metabolism
Reproducibility of Results
Methyltransferases metabolism
Nuclear Proteins metabolism
RNA Precursors metabolism
RNA Processing, Post-Transcriptional drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 6
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22195017
- Full Text :
- https://doi.org/10.1371/journal.pone.0029174