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Theranostic effect of serial manganese-enhanced magnetic resonance imaging of human embryonic stem cell derived teratoma.
- Source :
-
Magnetic resonance in medicine [Magn Reson Med] 2012 Aug; Vol. 68 (2), pp. 595-9. Date of Electronic Publication: 2011 Dec 21. - Publication Year :
- 2012
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Abstract
- Although human embryonic stem cell (hESC) hold therapeutic potential, teratoma formation has deterred clinical translation. Manganese (Mn(2+)) enters metabolically active cells through voltage-gated calcium channels and subsequently, induces T(1) shortening. We hypothesized that serial manganese-enhanced MRI would have theranostic effect to assess hESC survival, teratoma formation, and hESC-derived teratoma reduction through intracellular accumulation of Mn(2+). Firefly luciferase transduced hESCs (hESC-Lucs) were transplanted into severe combined immunodeficient mouse hindlimbs to form teratoma. The chemotherapy group was injected with MnCl(2) intraperitoneally three times a week. The control group was given MnCl(2) only prior to manganese-enhanced MRI. Longitudinal evaluation by manganese-enhanced MRI and bioluminescence imaging was performed. The chemotherapy group showed significant reduction in the teratoma volume and luciferase activity at weeks 6 and 8. Histology revealed increased proportion of dead cells and caspase 3 positive cells in the chemotherapy group. Systemic administration of MnCl(2) enabled simultaneous monitoring and elimination of hESC-derived teratoma cells by higher intracellular accumulation of Mn(2+).<br /> (Copyright © 2011 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Antineoplastic Agents therapeutic use
Cell Differentiation drug effects
Cell Line, Tumor
Contrast Media therapeutic use
Humans
Mice
Treatment Outcome
Chlorides therapeutic use
Embryonic Stem Cells pathology
Magnetic Resonance Imaging methods
Manganese Compounds therapeutic use
Teratoma drug therapy
Teratoma pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1522-2594
- Volume :
- 68
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Magnetic resonance in medicine
- Publication Type :
- Academic Journal
- Accession number :
- 22190225
- Full Text :
- https://doi.org/10.1002/mrm.23262