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Analysis of chromatin structure in plant cells.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 833, pp. 201-23. - Publication Year :
- 2012
-
Abstract
- A vast body of evidence in the literature indicates that nucleosomes can act as barriers to transcriptional initiation. The nucleosome at the promoter inhibits association of transcription factors disallowing active transcription of the gene. We have found a nucleosome on tobacco pathogenesis-related gene-1a (PR-1a) core promoter and mapped its boundaries and extension to find its span. The nucleosome covers the TATA box and Inr region of the core promoter and gets disassembled upon induction. Prior to its removal, modifications (i.e., acetylation and methylation of histones) occur at the nucleosome, proving a role of epigenetic modifications in transcriptional regulation. We summarize here various methodologies to analyze promoter chromatin structure in plants using the PR-1a core promoter as an example.
- Subjects :
- Antibodies immunology
Arabidopsis cytology
Arabidopsis metabolism
Base Sequence
Chromatin Immunoprecipitation
DNA Primers metabolism
DNA, Plant isolation & purification
Electrophoresis, Polyacrylamide Gel
Histones metabolism
Micrococcal Nuclease metabolism
Molecular Sequence Data
Nucleosomes metabolism
Polymerase Chain Reaction
Protein Binding
Protein Processing, Post-Translational
Time Factors
Chromatin chemistry
Molecular Biology methods
Plant Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 833
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 22183596
- Full Text :
- https://doi.org/10.1007/978-1-61779-477-3_13