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Ultrastructure and molecular phylogeny of thaumatomonads (Cercozoa) with emphasis on Thaumatomastix salina from Oslofjorden, Norway.
- Source :
-
Protist [Protist] 2012 Jul; Vol. 163 (4), pp. 560-73. Date of Electronic Publication: 2011 Dec 16. - Publication Year :
- 2012
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Abstract
- A culture of Thaumatomastix was isolated from a sediment sample collected in Oslofjorden and established as a monospecific strain (UIO286). Based on this culture, light and transmission electron microscopy and phylogenetic analyses were carried out. Thaumatomastix species are confined within the order Thaumatomonadida of the class Imbricatea and phylum Cercozoa. They are heterotrophic and their cell bodies are covered with silica scales. Observations of thin sections as well as whole mounts indicate that the morphology and ultrastructure of UIO286 is identical to T. salina, which was initially described from salt pools in Denmark. Detailed examination revealed some new features such as the presence of pseudopodia and silica deposition vesicles producing spine scales. The phylogeny presented here includes ribosomal DNA sequences from both imbricatean cultures and environmental samples. The 18S rDNA phylogenetic tree suggests that (i) Thaumatomastix is paraphyletic within the Thaumatomonadida clade, (ii) there is no close affinity between T. salina and other cultured and sequenced strains, but it is closely related to a sequence obtained from environmental DNA; we propose the present strain to serve as a reference culture of Thaumatomastix species and T. salina. Further, we discuss the distribution, habitats, and evolution of scale formation among euglyphids and thaumatomonads.<br /> (Copyright © 2011 Elsevier GmbH. All rights reserved.)
- Subjects :
- Cercozoa cytology
Cercozoa genetics
Cluster Analysis
DNA, Protozoan chemistry
DNA, Protozoan genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Genes, rRNA
Heterotrophic Processes
Microscopy
Molecular Sequence Data
Norway
Phylogeny
RNA, Protozoan genetics
RNA, Ribosomal, 18S genetics
Sequence Analysis, DNA
Cercozoa classification
Cercozoa isolation & purification
Geologic Sediments parasitology
Seawater parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0941
- Volume :
- 163
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Protist
- Publication Type :
- Academic Journal
- Accession number :
- 22177453
- Full Text :
- https://doi.org/10.1016/j.protis.2011.10.007