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[Improved method for the demonstration of the crystalline lens cellular structures].

Authors :
Korsakova NV
Sergeeva VE
Source :
Morfologiia (Saint Petersburg, Russia) [Morfologiia] 2011; Vol. 140 (4), pp. 65-6.
Publication Year :
2011

Abstract

A new method for the demonstration of the lens epithelial cells and fibers in the eye of the experimental animals (rats) is described. The method proposed is not laborious and is simple in utilization. It is based on the intravital administration of methylene blue solution to the animal with the subsequent supravital crystalline lens staining at 37 degreesC. The material was then embedded into paraffin, and the sections were stained with hematoxylin-eosin. The sections obtained using the proposed method, give an opportunity for the detailed demonstration of the morphological features of the lens cells.

Details

Language :
Russian
ISSN :
1026-3543
Volume :
140
Issue :
4
Database :
MEDLINE
Journal :
Morfologiia (Saint Petersburg, Russia)
Publication Type :
Academic Journal
Accession number :
22171437