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CD4(+) CD25(+) FoxP3(+) T regulatory cells in subjects responsive or unresponsive to hepatitis B vaccination.
- Source :
-
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2011 Nov; Vol. 36 (11), pp. 1046-51. - Publication Year :
- 2011
-
Abstract
- Objective: To determine CD4(+) CD25(+) T regulatory cells (Tregs), forkhead box P3 (FoxP3) mRNA expression and levels of cytokines secreted by peripheral blood mononuclear cells (PBMCs) in individuals responsive or unresponsive to hepatitis B (HB) vaccination, and to explore the relationships between immune response and immune regulatory cells or cytokines.<br />Methods: Based on the antibody against hepatitis B surface antigen (HBsAg) after HB vaccination, the CD4(+) CD25(+) Tregs frequencies in PBMCs from 18 responders, 22 nonresponders and 10 non-immunized healthy controls were analyzed by flow cytometry. The expression of FoxP3 mRNA in PBMCs with or without stimulation of phytohemagglutinin (PHA)and HBsAg was analyzed by real-time quantitative PCR. Levels of IL-4, IL-12, IL-18, and IFN-γ secreted by PBMCs after PHA and HBsAg stimulation were analyzed by enzyme-linked immunosorbent assay.<br />Results: The ratio of CD4(+) CD25(+) Tregs to CD4(+) T cells in the nonresponders was markedly higher than that in the responders (P<0.05), but lower than that in the controls (P<0.01). FoxP3 was differentially expressed among the responders, nonresponders, and controls in PBMCs before and after PHA and HBsAg stimulation, and nonresponders had the highest FoxP3 mRNA expression (P<0.05 or P<0.01). The content of IFN-γ by PBMCs after PHA and HBsAg stimulation was markedly lower in the nonresponders as compared with the controls and responders (P<0.05). However, there were no significant differences in the levels of IL-18, IL-4, and IL-12 from PBMCs after PHA and HBsAg stimulation between the responders and controls as well as the nonresponders (P>0.05).<br />Conclusion: CD4(+) CD25(+) FoxP3(+) Tregs may be involved in the negative regulation of responses to hepatitis B vaccination. Immunologic non-responses to hepatitis B vaccination may be related to IFN-γ hyposecretion in PBMCs.
- Subjects :
- Adolescent
Antibody Formation
Case-Control Studies
Female
Forkhead Transcription Factors genetics
Forkhead Transcription Factors metabolism
Hepatitis B immunology
Humans
Interferon-gamma metabolism
Interleukins metabolism
Leukocytes, Mononuclear immunology
Leukocytes, Mononuclear metabolism
Male
RNA, Messenger genetics
RNA, Messenger metabolism
T-Lymphocytes, Regulatory cytology
Young Adult
Forkhead Transcription Factors immunology
Hepatitis B prevention & control
Hepatitis B Vaccines immunology
T-Lymphocytes, Regulatory immunology
Vaccination
Subjects
Details
- Language :
- English
- ISSN :
- 1672-7347
- Volume :
- 36
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 22169715
- Full Text :
- https://doi.org/10.3969/j.issn.1672-7347.2011.11.003