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Effect of progesterone on the in vitro response of peripheral blood mononuclear cells stimulated by Escherichia coli in mares.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 2012 May; Vol. 74 (5), pp. 629-32. Date of Electronic Publication: 2011 Dec 13. - Publication Year :
- 2012
-
Abstract
- Escherichia coli(E. coli) isolated from the uterus of a Thoroughbred mare with bacterial endometritis was used to evaluate the effect of progesterone (P(4)) on the immune response of mares. Peripheral blood mononuclear cells (PBMCs) were collected from 10 nonpregnant clinically healthy adult mares (range, 4-12 years) during diestrus, four Thoroughbreds and six Hokkaido native horses. Cell proliferation and expression of cytokine mRNA, including interferon (IFN)-γ, tumor necrosis factor (TNF)-α and interleukin (IL)-10, of PBMCs stimulated with E. coli and P(4) were examined in vitro. P(4) was shown to have significantly inhibited E. coli induced proliferation and expression of IFN-γ in PBMCs. These results indicate that P(4) inhibits the immune response to E. coli in mares.
- Subjects :
- Animals
Cell Proliferation
Cells, Cultured
Cytokines genetics
Cytokines metabolism
Female
Gene Expression Regulation
Leukocytes, Mononuclear cytology
RNA, Messenger genetics
RNA, Messenger metabolism
Escherichia coli physiology
Horses blood
Leukocytes, Mononuclear drug effects
Leukocytes, Mononuclear immunology
Progesterone pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1347-7439
- Volume :
- 74
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 22167103
- Full Text :
- https://doi.org/10.1292/jvms.11-0364