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Effect of progesterone on the in vitro response of peripheral blood mononuclear cells stimulated by Escherichia coli in mares.

Authors :
Maeda Y
Ohtsuka H
Tomioka M
Tanabe T
Nambo Y
Uematsu H
Oikawa MA
Source :
The Journal of veterinary medical science [J Vet Med Sci] 2012 May; Vol. 74 (5), pp. 629-32. Date of Electronic Publication: 2011 Dec 13.
Publication Year :
2012

Abstract

Escherichia coli(E. coli) isolated from the uterus of a Thoroughbred mare with bacterial endometritis was used to evaluate the effect of progesterone (P(4)) on the immune response of mares. Peripheral blood mononuclear cells (PBMCs) were collected from 10 nonpregnant clinically healthy adult mares (range, 4-12 years) during diestrus, four Thoroughbreds and six Hokkaido native horses. Cell proliferation and expression of cytokine mRNA, including interferon (IFN)-γ, tumor necrosis factor (TNF)-α and interleukin (IL)-10, of PBMCs stimulated with E. coli and P(4) were examined in vitro. P(4) was shown to have significantly inhibited E. coli induced proliferation and expression of IFN-γ in PBMCs. These results indicate that P(4) inhibits the immune response to E. coli in mares.

Details

Language :
English
ISSN :
1347-7439
Volume :
74
Issue :
5
Database :
MEDLINE
Journal :
The Journal of veterinary medical science
Publication Type :
Academic Journal
Accession number :
22167103
Full Text :
https://doi.org/10.1292/jvms.11-0364