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Isolation and identification of a new tetrodotoxin-producing bacterial species, Raoultella terrigena, from Hong Kong marine puffer fish Takifugu niphobles.
- Source :
-
Marine drugs [Mar Drugs] 2011; Vol. 9 (11), pp. 2384-2396. Date of Electronic Publication: 2011 Nov 14. - Publication Year :
- 2011
-
Abstract
- Puffer fish, Takifugu niphobles, collected from the Hong Kong coastal waters were screened for tetrodotoxin-producing bacteria. A Gram-negative, non-acid-fast, non-sporing and rod shaped bacterial strain (designated as gutB01) was isolated from the intestine of the puffer fish and was shown to produce tetrodotoxin (TTX). Based on the Microbial Identification (MIDI) and 16S-23S rDNA internal transcribed spacer (ITS) phylogenetic analysis, the strain was identified as Raoultella terrigena. The TTX production ability of the strain was confirmed by mouse bioassay, ELISA and mass spectrometry (MALDI-TOF). Our results reiterate that the TTX found in puffer fish was likely produced by the associated bacteria and TTX are widely produced amongst a diversity of bacterial species.
- Subjects :
- Animals
Biological Assay
Enzyme-Linked Immunosorbent Assay
Hong Kong
Intestines microbiology
Klebsiella metabolism
Mass Spectrometry
Mice
Mice, Inbred ICR
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tetrodotoxin toxicity
Klebsiella isolation & purification
Tetraodontiformes microbiology
Tetrodotoxin isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1660-3397
- Volume :
- 9
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Marine drugs
- Publication Type :
- Academic Journal
- Accession number :
- 22163191
- Full Text :
- https://doi.org/10.3390/md9112384